Engineering pancreatic islets
Engineering pancreatic islets
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DOI：
10.1007/s004240000251
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发表时间：
2000-05
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影响因子：
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通讯作者：
B. Soria;E. Andreu;G. Berná;E. Fuentes;A. Gil;T. León‐Quinto;F. Martín;E. Montanya;A. Nadal;J. Reig;C. Ripoll;E. Roche;J. Sánchez-Andrés;J. Segura
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文献类型：
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作者：
B. Soria;E. Andreu;G. Berná;E. Fuentes;A. Gil;T. León‐Quinto;F. Martín;E. Montanya;A. Nadal;J. Reig;C. Ripoll;E. Roche;J. Sánchez-Andrés;J. Segura
胰岛是控制血糖稳态的神经内分泌器官。异质细胞群（β、α、δ和PP细胞）的精确相互作用导致平衡激素（分别为胰岛素、胰高血糖素、生长抑素和胰多肽）的微调释放。在详细了解这种行为背后的生理基础的前提下，可以推断出两条调查路线：生成计算和操作模型来解释和预测这种行为，以及工程胰岛细胞来重建胰腺内分泌功能。虽然前者是由新的计算策略，使生物药理学家的可能性建模系统中出现新的“涌现”的属性，后者是受益于分子，细胞和发育生物学家实现的有用的工具和战略知识。这包括使用肿瘤细胞系，工程胰岛细胞前体，分化，再生和生长机制的知识，以及最后，人类组织的治疗性克隆。获得对这些过程的基础的深刻生理学理解有助于工程化新的胰岛。
Pancreatic islets are neuroendocrine organs that control blood glucose homeostasis. The precise interplay of a heterogeneous group of cell populations (β, α, δ and PP cells) results in the fine-tuned release of counterbalanced hormones (insulin, glucagon, somatostatin and pancreatic polypeptide respectively). Under the premises of detailed knowledge of the physiological basis underlying this behaviour, two lines of investigation might be inferred: generating computational and operational models to explain and predict this behaviour and engineering islet cells to reconstruct pancreatic endocrine function. Whilst the former is being fuelled by new computational strategies, giving biophysicists the possibility of modelling a system in which new "emergent" properties appear, the latter is benefiting from the useful tools and strategic knowledge achieved by molecular, cell and developmental biologists. This includes using tumour cell lines, engineering islet cell precursors, knowledge of the mechanisms of differentiation, regeneration and growth and, finally, therapeutic cloning of human tissues. Gaining deep physiological understanding of the basis governing these processes is instrumental for engineering new pancreatic islets.