De novo genetic variation associated with retrotransposon activation, genomic rearrangements and trait variation in a recombinant inbred line population of Brassica napus derived from interspecific hybridization with Brassica rapa.
De novo genetic variation associated with retrotransposon activation, genomic rearrangements and trait variation in a recombinant inbred line population of Brassica napus derived from interspecific hybridization with Brassica rapa.
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DOI：
10.1111/j.1365-313x.2011.04679.x
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发表时间：
2011-10
期刊：
影响因子：
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通讯作者：
J. Zou;D. Fu;Huihui Gong;W. Qian;W. Xia;J. Pires;Ruiyuan Li;Y. Long;A. Mason;Tae-Jin Yang-Tae
中科院分区：
文献类型：
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作者：
J. Zou;D. Fu;Huihui Gong;W. Qian;W. Xia;J. Pires;Ruiyuan Li;Y. Long;A. Mason;Tae-Jin Yang-Tae
种间杂交是一种重要的进化力量，也是作物育种的有力手段。通过两轮种间杂交，将甘蓝型油菜(A(N)A(N)C(N)C(N))的AA亚基因组部分替换为甘蓝型油菜(A(R))基因组，获得了一种新的导入型甘蓝型油菜(A(R)A(R)C(N)C(N))。在本研究中，我们构建了一个新的导入型油菜重组自交系群体。利用微卫星标记、内含子标记和反转录转座子标记对该实验群体进行了遗传作图、遗传图谱比较和特异标记克隆分析。还记录了产量相关性状，以确定数量性状基因座(QTL)。在该群体中观察到了一系列新的基因组变化，包括简单序列重复(SSR)突变、染色体重排和反转录转座子激活。这些变化大多发生在种间杂交之后，即基因组稳定和实验品系产生的早期阶段。这些新的基因组改变对导入甘蓝型油菜的产量相关性状的影响甚至比从甘蓝型油菜A(R)亚基因组单独导入的等位基因更大，这表明由种间杂交引起的基因组变化在基因组进化和作物改良中都具有非常重要的意义。
Interspecific hybridization is a significant evolutionary force as well as a powerful method for crop breeding. Partial substitution of the AA subgenome in Brassica napus (A(n) A(n) C(n) C(n) ) with the Brassica rapa (A(r) A(r) ) genome by two rounds of interspecific hybridization resulted in a new introgressed type of B. napus (A(r) A(r) C(n) C(n) ). In this study, we construct a population of recombinant inbred lines of the new introgressed type of B. napus. Microsatellite, intron-based and retrotransposon markers were used to characterize this experimental population with genetic mapping, genetic map comparison and specific marker cloning analysis. Yield-related traits were also recorded for identification of quantitative trait loci (QTLs). A remarkable range of novel genomic alterations was observed in the population, including simple sequence repeat (SSR) mutations, chromosomal rearrangements and retrotransposon activations. Most of these changes occurred immediately after interspecific hybridization, in the early stages of genome stabilization and derivation of experimental lines. These novel genomic alterations affected yield-related traits in the introgressed B. napus to an even greater extent than the alleles alone that were introgressed from the A(r) subgenome of B. rapa, suggesting that genomic changes induced by interspecific hybridization are highly significant in both genome evolution and crop improvement.