The influence of electro-acupuncture on neural plasticity in acute cerebral infarction
The influence of electro-acupuncture on neural plasticity in acute cerebral infarction
复制标题
电针对急性脑梗死神经可塑性的影响
DOI：
10.1179/174313208x325182
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发表时间：
2008-11-01
影响因子：
1.9
通讯作者：
Wang, Jing X.
中科院分区：
文献类型：
--
作者：
Ren, Li;Zhang, Wu A.;Wang, Jing X.
摘要 目的：观察电针对树突棘和肝配蛋白A5的影响，探讨电针对急性脑缺血性梗死后神经可塑性的作用。方法：采用大脑中动脉闭塞（MCAO）电凝对侧法建立局灶性急性脑缺血模型。 90只雄性Sprague-Dawley大鼠随机分为假手术（SO）组、MCAO组和电针治疗（ET）组。采用高尔基体染色、双重免疫荧光法和RT-PCR检测缺血后第1、2、4周末的树突棘密度、ephrin-A5表达模式以及电针对其的影响。结果：缺血后MCAO组树突棘密度显着降低（p<0.01）。相应时间段内 ET 组的树突棘密度升高（p<0.01），并且各 ET 组之间的树突棘密度升高。第四周结束时比之前更高 (p<0.05)。 ephrin-A5信号主要在神经元细胞质中检测到，MCAO组和ET组mRNA表达量较SO组增加（p<0.01）。 ET组第1周mRNA表达量明显高于MCAO组(p<0.01)。 ET组中ephrin-A5 mRNA表达随时间延长而下调(p<0.01)。结论：电针治疗改善急性脑缺血大鼠梗死周围大脑皮层神经可塑性可能是通过调节ephrin-A5的表达而实现的。电针治疗急性脑缺血可能存在一个时间窗。
Abstract Objective: To observe the effect of electro-acupuncture (EA) on dendritic spine and ephrin-A5 and to investigate the action of EA on neural plasticity after acute cerebral ischemic infarction. Methods: Focal acute cerebral ischemia model was established by middle cerebral artery occlusion (MCAO) with electrocoagulation contralateral method. Ninety male Sprague–Dawley rats were randomly divided into sham operation (SO) group, MCAO group and EA treatment (ET) group. Golgi dying, double immunofluorescence method and RT-PCR were used to detect dendritic spine density, expression patterns of ephrin-A5 and the effect of EA on them at the end of the first, second and fourth week after ischemia. Results: The dendritic spine density in MCAO group significantly decreased after ischemia (p<0.01). The dendritic spine density was raised in ET group in the corresponding time period (p<0.01) and among the ET groups. It was higher at the end of the fourth week than before (p<0.05). The signal of ephrin-A5 was detected mainly in neuron cytoplasm, and the mRNA expression in MCAO group and ET group increased compared to that in SO group (p<0.01). The mRNA expression in ET group at the first week was much higher than that in MCAO group (p<0.01). In ET groups, the mRNA expression of ephrin-A5 was down-regulated along with the time going (p<0.01). Conclusion: It is possibly the regulation of the ephrin-A5 expression by which EA treatment improves the neural plasticity at the peri-infarct cerebral cortex in acute cerebral ischemia rat. There may be a time window in EA treatment for acute cerebral ischemia.