Estimating benzimidazole residues in thatch and turfgrass by bioassay
Estimating benzimidazole residues in thatch and turfgrass by bioassay
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DOI：
10.1002/(sici)1096-9063(199602)46:2
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发表时间：
1996-02-01
期刊：
影响因子：
--
通讯作者：
Hsiang, T
中科院分区：
文献类型：
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作者：
Liu, LX;Hsiang, T
利用苯并咪唑敏感的扩展青霉菌（Penicillium expansum Link），建立了琼脂、茅草琼脂和草坪琼脂微球生物测定法，以检测茅草和草坪剪草中苯菌灵（1-（丁基氨基甲酰基）苯并咪唑-2-基氨基甲酸甲酯）的主要降解产物苯并咪唑-2-基氨基甲酸甲酯（MBC）。这些生物测定用于估计生物可利用的杀真菌剂的量，因此，通过从所施用的量中减去保持结合和生物不可利用的量。定量限为0.5 mg kg（-1）。从19.9%至93.2%的所施加的杀真菌剂被结合的茅草和46.2%至56.9%被结合到草坪草剪下的取决于所使用的浓度。体外降解研究表明，MBC在23摄氏度的非灭菌茅草中的半衰期约为2.5周。
Bioassays using pellets of agar, thatch-agar and turfgrass-agar were developed using benzimidazole-sensitive Penicillium expansum Link, to detect the fungicide methyl benzimidazol-2-ylcarbamate (MBC) which is the major fungitoxic degradation product of benomyl [methyl 1-(butylcarbamoyl)benzimidazol-2-ylcarbamate] in thatch and turfgrass clippings. These bioassays were used to estimate the amount of fungicide that was biologically available and hence, by subtraction from that applied, the amount that remained bound and biologically unavailable. The limit of quantitation was 0.5 mg kg(-1). From 19.9% to 93.2% of the applied fungicide was bound by thatch and 46.2% to 56.9% was bound to turfgrass clippings depending on the concentrations used. In-vitro degradation studies showed that MBC had a half life of approximately 2.5 weeks at 23 degrees C in non-sterilized thatch.