Immunohistochemical analysis of cytochrome P-450 17 alpha-hydroxylase in pig adrenal cortex, testis and ovary.
Immunohistochemical analysis of cytochrome P-450 17 alpha-hydroxylase in pig adrenal cortex, testis and ovary.
复制标题
猪肾上腺皮质、睾丸和卵巢中细胞色素 P-450 17 α-羟化酶的免疫组织化学分析。
DOI：
10.1016/0303-7207(89)90006-3
复制
发表时间：
1989
影响因子：
4.1
通讯作者：
Sasano,N
中科院分区：
文献类型：
--
作者：
Sasano,H;Mason,JI;Sasano,N
使用从新生猪睾丸中纯化的针对 P-45017α 的特异性抗体，通过生物素-链霉亲和素方法，在猪肾上腺皮质、睾丸和卵巢中对类固醇 17α-羟基化 (P-45017α) 特异性的细胞色素 P-450 进行免疫组织化学观察。在肾上腺皮质中，P-45017α存在于束带和网状带中，而在肾小球带中未观察到免疫反应性，证实肾小球带中不存在17-羟基化类固醇合成。在睾丸中，P-45017α 仅存在于 Leydig 细胞中，而生精小管中不存在免疫反应性。在卵巢中，仅在内卵泡膜中观察到免疫反应性，而在卵泡颗粒膜中未观察到免疫反应性。在检查的组织中，间质细胞的免疫反应性相对强度最大，而内膜细胞、外束状细胞以及内束状和网状细胞的免疫反应性相对强度逐渐减弱。
CytochromeP-450 specific for steroid 17α-hydroxylation (P-45017α) was immunohistochemically observed in pig adrenal cortex, testis and ovary by the biotin-streptavidin method using a specific antibody againstP-45017αpurified from neonatal pig testis. In the adrenal cortex,P-45017αwas present in the zona fasciculata and reticularis while no immunoreactivity was observed in the zona glomerulosa, confirming the absence of 17-hydroxylated steroid synthesis in the zona glomerulosa. In the testis,P-45017αwas present exclusively in Leydig cells and immunoreactivity was absent in seminiferous tubules. In the ovary, immunoreactivity was observed only in the theca interna but not in the membrana granulosa of follicles. Among the tissues examined, the relative intensity of immunoreactivity was greatest in the Leydig cells, and progressively less in theca interna cells, outer fasciculata cells and inner fasciculata and reticularis cells.