[Identification and determination of major constituents in Polygonum cuspidatum Sieb. et Zucc. by high performance liquid chromatography/electrospray ionization-ion trap-time-of-flight mass spectrometry].
[Identification and determination of major constituents in Polygonum cuspidatum Sieb. et Zucc. by high performance liquid chromatography/electrospray ionization-ion trap-time-of-flight mass spectrometry].
复制标题
DOI：
--
复制
发表时间：
2009-07
期刊：
影响因子：
--
通讯作者：
Jing Dong;Hong Wang;L. Wan;Y. Hashi;Shizhong Chen
中科院分区：
文献类型：
--
作者：
Jing Dong;Hong Wang;L. Wan;Y. Hashi;Shizhong Chen
建立了高效液相色谱/电喷雾电离-离子阱飞行时间质谱（HPLC/ESI-IT-TOF MS）分析虎杖中多种成分的简便方法。调查。对大黄素、大黄酚、大黄素、大黄素、芦荟大黄素、大黄素等6种化学标准品进行了系统研究，并总结了它们的破碎途径。虎杖甲醇提取物的研究。调查。HPLC/ESI-QIT-TOF MS系统在负离子模式下分离分析。在6种化学标准和相关参考文献的裂解途径上，共鉴定或初步表征了10种成分。这些成分主要是蒽醌类、二苯乙烯类、金针桃素及其衍生物，包括白藜芦醇苷、聚datatin、大黄素-8- o -葡萄糖苷、白藜芦醇、金针桃素-8- o -葡萄糖苷、大黄素-1- o -葡萄糖苷、金针桃素-8- o -（6'-乙酰基）葡萄糖苷（新发现）、金针桃素-8- o -（6'-乙酰基）葡萄糖苷、金针桃素-8- o -（6'-乙酰基）葡萄糖苷（新发现）和大黄素。采用HPLC/ESI-IT-TOF MS提供中草药成分的化学信息，使鉴定结果更有说服力，是一种非常简单的方法。
A facile method using high performance liquid chromatography/electrospray ionization-ion trap-time-of-flight mass spectrometry (HPLC/ESI-IT-TOF MS) has been established for the analysis of multiple constituents in Polygonum cuspidatum Sieb. et Zucc. Six chemical standards including emodin, chrysophanol, physcion, rhein, aloe-emodin and polydatin were studied systematically and their fragmentation pathways were concluded. The methanol extract of Polygonum cuspidatum Sieb. et Zucc. was separated and analyzed by HPLC/ESI-QIT-TOF MS system in negative ion mode. A total of 10 constituents were identified or tentatively characterized with supporting results on the fragmentation pathways of 6 chemical standards and relative references. These constituents are mainly anthraquinones, stilbenes, torachryson and their derivatives, including resveratroloside, polydatin, emodin-8-O-glucoside, resveratrol, torachryson-8-O-glucoside, emodin-1-O-glucoside, torachryson-8-O-(6'-acetyl)glucoside (newly discovered), physcion-8-O-glucoside, physcion-8-O-(6'-acetyl)glucoside (newly discovered) and emodin. It is an extremely simple way by using HPLC/ESI-IT-TOF MS to provide chemical information concerning the constituents in herbal medicines and making the identification results more convinced.