Bioactive C21 Steroidal Glycosides from the Roots of Cynanchum otophyllum That Suppress the Seizure-like Locomotor Activity of Zebrafish Caused by Pentylenetetrazole
Bioactive C21 Steroidal Glycosides from the Roots of Cynanchum otophyllum That Suppress the Seizure-like Locomotor Activity of Zebrafish Caused by Pentylenetetrazole
复制标题
徐长卿根中的生物活性 C21 甾体苷可抑制戊四唑引起的斑马鱼癫痫样运动活动
DOI：
10.1021/np501058b
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发表时间：
2015-07-01
影响因子：
5.1
通讯作者：
Zhao, Wei-Min
中科院分区：
文献类型：
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作者：
Li, Jin-Long;Zhou, Juan;Zhao, Wei-Min
从白首乌Cynanchum otophyllum的根中分离得到6个新的C-21甾体苷类化合物，即Cynotophyllosides A-F（1-6）和16个已知化合物。新化合物的结构经波谱分析和化学方法鉴定。三种主要成分耳叶苷F（15）、耳叶苷B（17）和rostratamine 3-O-β-D-吡喃夹竹桃糖基-（1 β-D-吡喃夹竹桃糖基-（1-> 4）-β-D-吡喃环己糖基（1 -> 4）-β-D-吡喃环己糖基）抑制了戊四唑引起的斑马鱼的类神经元样运动活动。初步的结构-活性关系研究表明，具有C-12酯基（ikemaoyl > cinnamoyl > hydroxy > p-hydroxybenzoyl）和C-3糖链（由三个2，6-二脱氧糖单元组成）的藜芦烯骨架对于这种抑制活性是必需的。
Six new C-21 steroidal glycosides, cynotophyllosides A-F (1-6), together with 16 known compounds, were isolated from the roots of Cynanchum otophyllum. The structures of the new compounds were elucidated by spectroscopic analysis and chemical methods. The three major components, otophylloside F (15), otophylloside B (17), and rostratamine 3-O-beta-D-oleandropyranosyl-(1 beta-D-oleandropyranosyl-(1 -> 4)-beta-D-cymaropyranosyl (1 -> 4)-beta-D-cymaropyranoside), suppressed the seizure-like locomotor activity caused by pentylenetetrazole in zebrafish. Preliminary structure-activity relation studies revealed that a pregnene skeleton with a C-12 ester group (ikemaoyl > cinnamoyl > hydroxy > p-hydroxybenzoyl) and a C-3 sugar chain consisting of three 2,6-dideoxysaccharide units is essential for this suppressive activity.