In vitro cellular tropism of human B-lymphotropic virus (human herpesvirus-6)
In vitro cellular tropism of human B-lymphotropic virus (human herpesvirus-6)
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人 B 淋巴细胞病毒（人疱疹病毒 6）的体外细胞趋向性
DOI：
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发表时间：
1988
影响因子：
15.3
通讯作者：
R. Gallo
中科院分区：
文献类型：
--
作者：
P. Lusso;P. Markham;E. Tschachler;F. Di;Marzo Veronese;I. S. Zaki Salahuddin;D. Ablashi;S. Pahwa;K. Krohn;R. Gallo
我们研究了人B淋巴细胞嗜性病毒（HBLV）（也称为人疱疹病毒-6）在体外感染新鲜MN细胞从正常成人外周血，脐带血，骨髓，扁桃体，胸腺。所有来源的培养物均含有可感染的细胞，表现为特征性的放大、圆形、短寿命细胞，表达HBLV特异性标志物。详细的免疫学分析表明，这些细胞中的绝大多数表达T细胞相关抗原（即，CD 7、CD 5、CD 2、CD 4，以及在较小程度上，CD 8）。通过免疫荧光和放射免疫沉淀试验，在表面膜上未检测到CD 3抗原和TCR-α/β异源二聚体，但在HBLV感染细胞的细胞质内进行了鉴定。一部分HBLV感染的细胞群也表达CD 15和II类MHC DR抗原。通过免疫选择程序，可以表明在CD 3耗尽的未成熟T细胞群中含有一致比例的HBLV-感染细胞，而CD 2+细胞的耗尽完全消除了培养物的感染性。北方印迹分析证实了HBV感染细胞的T细胞来源，证明了全长TCR-α和β链mRNA的表达。除新鲜T细胞外，HBLV还能感染用IL- 2体外扩增的正常T淋巴细胞30 d以上。这些结果表明，HBLV是选择性的T细胞嗜性的过程中，在体外感染的正常单核细胞，因此可能直接参与T细胞相关的血液疾病的发病机制。特别是，鉴于体外对CD 4 + T淋巴细胞的致细胞病变作用，应考虑HBLV在免疫缺陷条件下的可能作用。
We investigated the cellular tropism of human B-lymphotropic virus (HBLV) (also designated Human Herpesvirus-6) in vitro by infecting fresh MN cells from normal human adult peripheral blood, umbilical cord blood, bone marrow, tonsil, and thymus. Cultures from all the sources examined contained infectable cells, as shown by the appearance of characteristic enlarged, round-shaped, short-lived cells expressing HBLV-specific markers. Detailed immunological analysis demonstrated that the vast majority of these cells expressed T cell-associated antigens (i.e., CD7, CD5, CD2, CD4, and to a lesser extent, CD8). The CD3 antigen and the TCR-alpha/beta heterodimer were not detectable on the surface membrane, but were identified within the cytoplasm of HBLV- infected cells, by both immunofluorescence and radioimmunoprecipitation assay. A proportion of the HBLV-infected cell population also expressed the CD15 and class II MHC DR antigens. By means of immunoselection procedures it was possible to show that a consistent proportion of HBLV- infectable cells were contained within the CD3-depleted immature T cell population, while the depletion of CD2+ cells completely abrogated the infectability of the cultures. Northern blot analysis confirmed the T cell origin of HBLV-infected cells, demonstrating the expression of full size TCR-alpha and -beta chain mRNA. In addition to fresh T cells, HBLV was able to infect normal T lymphocytes expanded in vitro with IL- 2 for greater than 30 d. These results indicate that HBLV is selectively T cell tropic in the course of the in vitro infection of normal mononuclear cells and may therefore be directly involved in the pathogenesis of T cell related hematological disorders. In particular, in light of the cytopathic effect exerted in vitro on CD4+ T lymphocytes, a possible role of HBLV in immune deficiency conditions should be considered.
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T 细胞肿瘤中 Leu-M1 抗原的表达。
DOI：
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发表时间：
1985
期刊：
The American journal of pathology
影响因子：
--
作者：
Wieczorek,R;Burke,JS;Knowles2nd,DM
通讯作者：
Knowles2nd,DM
3例患者B细胞淋巴瘤组织中人B淋巴细胞病毒（人疱疹病毒6）序列的检测。
影响因子：
11.4
作者：
Josephs,SF;Buchbinder,A;Streicher,HZ;Ablashi,DV;Salahuddin,SZ;Guo,HG;Wong-Staal,F;Cossman,J;Raffeld,M;Sundeen,J
通讯作者：
Sundeen,J
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DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Spits,H;Borst,J;Tax,W;Capel,PJ;Terhorst,C;deVries,JE
通讯作者：
deVries,JE
艾滋病患者 B 细胞淋巴瘤的发病机制。
影响因子：
20.3
作者：
Groopman,JE;Sullivan,JL;Mulder,C;Ginsburg,D;Orkin,SH;O'Hara,CJ;Falchuk,K;Wong-Staal,F;Gallo,RC
通讯作者：
Gallo,RC