RBMX is a novel hepatic transcriptional regulator of SREBP-1c gene response to high-fructose diet
RBMX is a novel hepatic transcriptional regulator of SREBP-1c gene response to high-fructose diet
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DOI：
10.1016/j.febslet.2006.12.014
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发表时间：
2007-01-23
期刊：
影响因子：
3.5
通讯作者：
Kashiwagi, Atsunori
中科院分区：
文献类型：
--
作者：
Nagai, Yoshio;Nishio, Yoshihiko;Kashiwagi, Atsunori
在啮齿类动物中，高果糖饮食诱导代谢紊乱，类似于代谢综合征。以前我们认为，在小鼠肝脏中，一个未鉴定的核蛋白结合到固醇调节元件（SRE）结合蛋白-1c（SREBP-1c）启动子区起着关键作用的反应，高果糖饮食。在这里，使用MALDI-TOF MASS技术，我们确定了一个X染色体连锁的RNA结合基序蛋白（RBMX）作为一个新的候选分子。在电泳迁移率变动分析中，抗RBMX抗体使果糖诱导的条带发生位移。RBMX在大鼠肝癌细胞上的过表达或抑制调节SREBP-1c启动子活性。RBMX可调控高果糖饮食小鼠肝脏SREBP-1c的表达。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
In rodents a high-fructose diet induces metabolic derangements similar to those in metabolic syndrome. Previously we suggested that in mouse liver an unidentified nuclear protein binding to the sterol regulatory element (SRE)-binding protein-1c (SREBP-1c) promoter region plays a key role for the response to high-fructose diet. Here, using MALDI-TOF MASS technique, we identified an X-chromosome-linked RNA binding motif protein (RBMX) as a new candidate molecule. In electrophoretic mobility shift assay, anti-RBMX antibody displaced the bands induced by fructose-feeding. Overexpression or suppression of RBMX on rat hepatoma cells regulated the SREBP-1c promoter activity. RBMX may control SREBP-1c expression in mouse liver in response to high-fructose diet. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.