Breakdown of self-incompatibility in tetraploid Lycopersicon peruvianum: inheritance and expression of S-related proteins
Breakdown of self-incompatibility in tetraploid Lycopersicon peruvianum: inheritance and expression of S-related proteins
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DOI：
10.1007/s001220050652
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发表时间：
1997-10-01
影响因子：
5.4
通讯作者：
Marcotrigiano, M
中科院分区：
文献类型：
--
作者：
Chawla, B;Bernatzky, R;Marcotrigiano, M
秘鲁番茄具有配子体自交不亲和性。我们获得了自交亲和四倍体L. peruvianum的S相关蛋白的表达和遗传进行了研究。SDS-PAGE分析表明，SC四倍体花柱与二倍体自交不亲和（SI）外植体花柱的S相关蛋白质图谱无明显差异。用克隆的S相关蛋白cDNA序列进行Southern杂交，确定该位点在这两个四倍体自交后代中的遗传。等位基因的比例，确定从DNA限制性片段的强度，与预测的比例是一致的，如果只有花粉轴承两个不同的等位基因是成功实现受精。获得的所有后代都具有每个等位基因的至少一个拷贝，并且没有发现对于任一等位基因完全纯合的个体，这表明具有两个相同等位基因的花粉粒被抑制。此外，在DNA水平上，后代中S相关蛋白的表达水平与等位基因剂量相关。我们证明，所观察到的自交不亲和性的四倍体是由S-相关蛋白的表达的改变。
Lycopersicon peruvianum displays gametophytic self-incompatibility (GSI). We have isolated self-compatible (SC) tetraploids of L. peruvianum from tissue-cultured leaves and have explored the expression and inheritance of their S-related proteins. The S-related protein profiles of styles of SC tetraploids were indistinguishable from the diploid self-incompatible (SI) explant source based on SDS-PAGE. All progeny obtained from self-fertilization of two tetraploids were SC. Cloned cDNA sequences of the S-related proteins were used to deter-mine the inheritance of this locus in these progeny through Southern hybridization. The allelic ratio, as determined from the intensity of DNA restriction fragments, was consistent with the predicted ratio if only pollen bearing two different alleles was successful in achieving fertilization. All progeny obtained had at least one copy of each allele, and individuals fully homozygous for either allele were not found, indicating that pollen grains bearing two identical alleles were inhibited. In addition, the level of expression of the S-related proteins in the progeny correlated with the allelic dosage at the DNA level. We demonstrate that the observed self-compatibility in the tetraploids was not caused by an alteration in the expression of S-related proteins.