Ozone oxidative preconditioning protects the rat kidney from reperfusion injury: The role of nitric oxide
Ozone oxidative preconditioning protects the rat kidney from reperfusion injury: The role of nitric oxide
复制标题
臭氧氧化预处理保护大鼠肾脏免受再灌注损伤：一氧化氮的作用
DOI：
10.1016/j.jss.2007.12.756
复制
发表时间：
2008-10-01
影响因子：
2.2
通讯作者：
Chen, Zhiyuan
中科院分区：
文献类型：
--
作者：
Chen, Hui;Xing, Bianzhi;Chen, Zhiyuan
背景资料。缺血再灌注(I/R)损伤是急性肾功能衰竭的主要原因之一，常见于肾脏外科或移植领域。已有研究表明，臭氧氧化预适应(OzoneOP)可减轻肾I/R损伤。本研究旨在验证OzoneOP对肾I/R损伤的保护作用与内源性NO有关的假说。材料和方法。在右肾切除大鼠模型中，麻醉大鼠造成45min的肾缺血。I/R损伤前给予OzoneOP(1 mg/kg)。分别于I/R损伤后24、48、72h处死大鼠，取血和肾组织。结果。OzoneOP可保护I/R所致的肾功能不全，增加肾组织一氧化氮(NO)的释放和肾组织一氧化氮合酶(eNOS、iNOS)的表达。相反，OzoneOP可显著抑制再灌流后肾组织内皮素-1的增加。结论。我们的研究结果表明，OzoneOP的保护作用与eNOS和iNOS表达增加后产生NO密切相关。臭氧治疗可能具有重要的临床意义，特别是考虑到移植前对肾脏的损害最小。(C)2008 Elsevier Inc.保留所有权利。
Background. Ischemia/reperfusion (I/R) injury, which is commonly seen in the field of renal surgery or transplantation, is a major cause of acute renal failure. Previous studies have shown that ozone oxidative preconditioning (OzoneOP) attenuated renal I/R injury. The objective of this study was to examine the hypothesis that protective effects of OzoneOP in renal I/R injury were associated with endogenous NO. Materials and methods. In a right-nephrectomized rat mode, anesthetized rats underwent 45 min of renal ischemia. OzoneOP (1 mg/kg) was administered before I/R injury. Rats were killed at 24,48, and 72 h after I/R injury and blood samples and renal tissues were obtained. Results. OzoneOP prevented the renal dysfunction induced by I/R and increased nitric oxide (NO) release and renal NO synthase (endothelial, eNOS, and inducible, iNOS) expression. In contrast, enhancement of endothelin-1 in the kidney after the reperfusion was markedly suppressed by OzoneOP. Conclusions. Our findings indicated that the protective effect of OzoneOP was closely related to the NO production following the increase in eNOS and iNOS expression. Ozone treatment may have important clinical implications, particularly in view of the minimizing renal damage before transplantation. (c) 2008 Elsevier Inc. All rights reserved.