Postischemic exercise decreases neurogenesis in the adult rat dentate gyrus
Postischemic exercise decreases neurogenesis in the adult rat dentate gyrus
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DOI：
10.1016/j.neulet.2006.09.040
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发表时间：
2006-11
影响因子：
2.5
通讯作者：
Y. Yagita;K. Kitagawa;Tsutomu Sasaki;Y. Terasaki;K. Todo;E. Omura-Matsuoka;M. Matsumoto;M. Hori
中科院分区：
文献类型：
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作者：
Y. Yagita;K. Kitagawa;Tsutomu Sasaki;Y. Terasaki;K. Todo;E. Omura-Matsuoka;M. Matsumoto;M. Hori
跑步运动促进正常成年人和老年人海马神经发生。然而，运动对缺血海马神经发生的影响尚不清楚。在这里，我们表明，跑步锻炼对缺血性和非缺血性大脑有不同的影响。年轻（3-4月龄）血压正常的Wistar大鼠用于本研究。方法：采用大鼠短暂性前脑缺血模型和假手术模型，于术后7天给予溴脱氧尿苷（BrdU）。免疫荧光双标显示，缺血区颗粒细胞层（GCL）和颗粒下带（SGZ）的BrdU标记细胞增多，约80%的BrdU标记细胞表达神经元标记物。为了评估跑步运动对神经发生的影响，在1天和14天后对这些区域中的BrdU标记细胞进行定量。在假手术大鼠，BrdU标记的细胞的数量显着增加（2.2倍），在SGZ和GCL在响应跑步运动。BrdU标记细胞的数量在缺血时增加，然而，它们在BrdU给药后14天减少，跑步运动加速了缺血大鼠BrdU标记细胞的减少。这些发现表明，跑步锻炼对缺血海马的神经发生有负面影响。这对于评估卒中后功能恢复的治疗方法可能是重要的。
Running exercise enhances neurogenesis in the normal adult and aged hippocampus. However, the effect of exercise on neurogenesis in the ischemic hippocampus is unclear. Here, we show that running exercise has different effects on ischemic and non-ischemic brain. Young (3–4-month-old) normotensive Wistar rats were used for this study. We administered bromodeoxyuridine (BrdU) to rats 7 days after the induction of transient forebrain ischemia or sham operation. BrdU-labeled cells were increased in the ischemic subgranular zone (SGZ) and granule cell layer (GCL) and double immunofluoresence showed approximately 80% of BrdU-labeled cells expressed neuronal markers. To assess the effect of running exercise on neurogenesis, BrdU-labeled cells in these regions were quantified after 1 day and 14 days. In sham-operated rats, the numbers of BrdU-labeled cells were significantly increased (2.2-fold) in the SGZ and GCL in response to running exercise. The numbers of BrdU-labeled cells were increased in response to ischemia, however, they were decreased 14 days after BrdU administration and running exercise accelerated the reduction in BrdU-labeled cells in ischemic rats. These findings suggest that running exercise has a negative effect on neurogenesis in the ischemic hippocampus. This may be important with respect to assessment of therapeutic approaches for functional recovery after stroke.