Genome shuffling of Lactobacillus delbrueckii mutant and Bacillus amyloliquefaciens through protoplasmic fusion for L-lactic acid production from starchy wastes
Genome shuffling of Lactobacillus delbrueckii mutant and Bacillus amyloliquefaciens through protoplasmic fusion for L-lactic acid production from starchy wastes
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DOI：
10.1016/j.biortech.2008.03.058
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发表时间：
2008-11-01
影响因子：
11.4
通讯作者：
Nampoothiri, K. Madhavan
中科院分区：
文献类型：
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作者：
John, Rojan P.;Gangadharan, Dhanya;Nampoothiri, K. Madhavan
目前的研究集中于通过德氏乳杆菌NCIM 2025的突变菌株和产生淀粉酶的非苛养解淀粉芽孢杆菌ATCC 23842的基因组改组来开发具有更好生长速率、低pH耐受性和良好生产力的非苛养乳酸产生菌株。第三次原生质体融合后，监测少数融合子的乳酸产量，并选择最佳融合子进行进一步研究。采用Plackett-Burman试验设计和响应面法对乳酸发酵的重要工艺参数进行了优化。所选融合子可直接利用液化木薯渣淀粉生产乳酸，且营养补充量少。在验证过程中，通过使用来自83 g/L木薯渣（淀粉含量50%w/w）的融合子接种物（3%，v/v），补充酵母提取物和蛋白胨（各0.2%，w/v）和缓冲剂（2%CaCO3，w/v），获得40 g/L乳酸（类似于96%淀粉转化为乳酸）。(C)2008爱思唯尔有限公司保留所有权利。
Current study was focused on the development of a non-fastidious lactic acid producing strain having better growth rate, low pH tolerance and good productivity by genome shuffling of a mutant strain of Lactobacillus delbrueckii NCIM 2025 and an amylase producing non-fastidious Bacillus amyloliquefaciens ATCC 23842. After the third cycle of the protoplast fusion, lactic acid production by few fusants was monitored and the best fusant was selected for further studies. Optimization of the important process parameters for lactic acid production was conducted using Plackett-Burman design and response Surface methodology. Selected fusant could utilize the liquefied cassava bagasse starch directly with minimum nutrient supplementation for lactic acid production. During validation, 40 g/L of lactic acid was obtained (similar to 96% conversion of starch to lactic acid) by using fusant inoculum (3%, v/v) from 83 g/L cassava bagasse (starch content 50% w/w) supplemented with yeast extract and peptone (0.2% each, w/v) and the buffering agent (2% CaCO3, w/v). (C) 2008 Elsevier Ltd. All rights reserved.