The reaction of H2S with oxidized thiols: Generation of persulfides and implications to H2S biology
The reaction of H2S with oxidized thiols: Generation of persulfides and implications to H2S biology
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DOI：
10.1016/j.abb.2011.09.015
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发表时间：
2011-12-15
影响因子：
3.9
通讯作者：
Fukuto, Jon M.
中科院分区：
文献类型：
--
作者：
Francoleon, Nestor E.;Carrington, Samantha J.;Fukuto, Jon M.
硫化氢是一种内源性产生的分子，具有许多报道的生理功能。虽然已经提出了几个生物学靶点，但其增强活性的生化机制尚未建立。因此，为了开始描述H2S的基本生物化学，我们已经研究了H2S与氧化硫醇和硫醇蛋白的反应，以确定是否发生过硫化物形成，是否稳定以及这如何影响蛋白质功能。我们发现过硫化物很容易产生，相对稳定，可以改变酶的活性。此外，我们已经开始开发的方法，在原位生成的过硫化物，以促进进一步研究这一潜在的重要物种。(C)2011 Elsevier Inc. All rights reserved.
Hydrogen sulfide is an endogenously generated molecule with many reported physiological functions. Although several biological targets have been proposed, the biochemical mechanisms by which it elicits activity are not established. Thus, in an effort to begin to delineate the fundamental biological chemistry of H2S, we have examined the reaction of H2S with oxidized thiols and thiol proteins in order to determine whether persulfide formation occurs, is stable and how this may affect protein function. We have found that persulfides are easily generated, relatively stable and can alter enzyme activity. Moreover, we have begun to develop methodology for in situ generation of persulfides to facilitate further study of this potentially important species. (C) 2011 Elsevier Inc. All rights reserved.