Analysis of sugars in glycoproteins by high-pressure liquid chromatography.
Analysis of sugars in glycoproteins by high-pressure liquid chromatography.
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通过高压液相色谱分析糖蛋白中的糖。
DOI：
10.1016/0003-2697(85)90248-9
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发表时间：
1985
影响因子：
2.9
通讯作者：
Jentoft,N
中科院分区：
文献类型：
--
作者：
Jentoft,N
本文建立了一种用甲醇分解过苯甲酰甲基糖苷，然后用反相高压液相色谱法分析糖蛋白和类似糖复合物的糖组成的方法。如上所述，该方法能够定量1- 10-nmol范围内的糖，同时进一步优化程序可以将可用灵敏度提高10倍或更高。糖衍生物的产率提高已通过将几个修改的原始甲醇分解程序。这与使用高压液相色谱而不是气相色谱分离糖衍生物一起，消除了对经验确定的摩尔响应比的需要。
A method for analyzing the carbohydrate composition of glycoproteins and similar glycoconjugates by methanolysis followed by revers-phase high-pressure liquid chromatography of the perbenzoylated methyl glycosides has been developed. As described, the method is capable of quantifying sugars in the 1- to 10-nmol range while further optimization of procedures may increase the usable sensitivity by a factor of 10 or greater. Improved yields of the sugar derivatives have been achieved by incorporating several modifications of the original methanolysis procedure. This, together with the use of high-pressure liquid chromatography rather than gas chromatography for separating the sugar derivatives, eliminates the need for empirically determined molar response ratios.