A study on the mechanism of antibody-dependent enhancement of feline infectious peritonitis virus infection in feline macrophages by monoclonal antibodies.
A study on the mechanism of antibody-dependent enhancement of feline infectious peritonitis virus infection in feline macrophages by monoclonal antibodies.
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DOI：
10.1007/bf01310476
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发表时间：
1991
影响因子：
2.7
通讯作者：
Koyama H
中科院分区：
文献类型：
--
作者：
Hohdatsu T;Nakamura M;Ishizuka Y;Yamada H;Koyama H
使用抗猫传染性腹膜炎病毒（FIPV）毒株79-1146的单克隆抗体（MAb）研究了FIPV感染猫巨噬细胞的增强作用。从猫肺和腹腔回收的贴壁细胞吞噬固定的红细胞，并形成Fc介导的红细胞。用病毒悬液和单克隆抗体的混合物通过肺泡巨噬细胞研究单克隆抗体对病毒感染的增强作用，并通过免疫荧光测定检测细胞内病毒抗原和孵育后上清液中感染性病毒的量。非中和性单克隆抗体识别病毒的膜蛋白（S）和跨膜蛋白（M），可增强病毒在巨噬细胞中的复制。即使在具有中和FIPV毒株79-1146的能力的MAb中，当它们被稀释时，一些单克隆抗体也增强病毒感染。用蛋白A预处理单克隆抗体可抑制增强。单克隆抗体的F（ab′）2片段可减弱增强。这些结果表明FIPV感染巨噬细胞的抗体依赖性增强（ADE）。FIPV 79-1146株在FIPV抗体阳性猫巨噬细胞中的复制比在抗体阴性猫巨噬细胞中的复制更强。
Enhancement of feline infectious peritonitis virus (FIPV) infection of feline macrophages was studied using monoclonal antibodies (MAbs) to the FIPV strain 79-1146. Adherent cells recovered from the feline lung and peritoneal cavity phagocytosed fixed red blood cells, and formed Fc-mediated rosettes. Enhancement of virus infection by MAb was investigated by inoculating alveolar macrophages with a mixtures of viral suspension and MAb, and examining the cells for intracellular viral antigen by the immunofluorescence assay and the amount of infectious virus in the supernatant fluid after incubation. The replication of FIPV in macrophages was enhanced by non-neutralizing MAbs recognizing peplomer protein (S) and transmembrane protein (M) of the virus. Even among the MAbs having the ability to neutralize FIPV strain 79-1146, some reversely enhanced virus infection when they were diluted. The enhancement was suppressed by pretreatment of the MAb with protein A. The enhancement was reduced by the use of F(ab′)2 fragment of MAb. These results demonstrated antibody-dependent enhancement (ADE) of FIPV infection in macrophage. The replication of FIPV 79-1146 strain in macrophages from FIPV antibody-positive cats was more enhanced than in those from antibody-negative cats.