Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation.
Identification and Characterization of an Aeromonas hydrophila Oligopeptidase Gene pepF Negatively Related to Biofilm Formation.
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与生物膜形成负相关的嗜水气单胞菌寡肽酶基因 pepF 的鉴定和表征
DOI：
10.3389/fmicb.2016.01497
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发表时间：
2016
影响因子：
5.2
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Du H;Pang M;Dong Y;Wu Y;Wang N;Liu J;Awan F;Lu C;Liu Y
细菌生物膜参与对复杂环境的适应，并负责持续的细菌感染。生物膜的形成是一个高度复杂的过程，在此过程中多种基因定期共同作用。在这项研究中，我们筛选EZ-Tn 5转座子突变库，以确定参与生物膜形成的气单胞菌的基因。共鉴定了24个生物膜相关基因，其中大部分编码与细胞结构、转录和翻译、基因调控、生长和代谢相关的蛋白质。突变株TM 90，其中编码寡肽酶F（pepF）的基因被干扰，显示出显着上调的生物膜形成相比，亲本菌株。TM 90菌落表型更小、更透明、更鲜艳。TM 90对HEp-2细胞的粘附能力明显增强。斑马鱼半数致死剂量（LD 50）测定表明，增强生物膜突变体TM 90是高度减毒相对于野生型菌株。综上所述，pepF基因首次被证明是生物膜形成的负因子，并参与了A.病原菌
Bacterial biofilms are involved in adaptation to complex environments and are responsible for persistent bacterial infections. Biofilm formation is a highly complex process during which multifarious genes work together regularly. In this study, we screened the EZ-Tn5 transposon mutant library to identify genes involved in biofilm formation of Aeromonas hydrophila. A total of 24 biofilm-associated genes were identified, the majority of which encoded proteins related to cell structure, transcription and translation, gene regulation, growth and metabolism. The mutant strain TM90, in which a gene encoding oligopeptidase F (pepF) was disturbed, showed significant upregulation of biofilm formation compared to the parental strain. The TM90 colony phenotype was smaller, more transparent, and splendent. The adhesive ability of TM90 to HEp-2 cells was significantly increased compared with the parental strain. Fifty percent lethal dose (LD50) determinations in zebrafish demonstrated that the enhanced-biofilm mutant TM90 was highly attenuated relative to the wild-type strain. In conclusion, the pepF gene is demonstrated for the first time to be a negative factor for biofilm formation and is involved in A. hydrophila pathogenicity.