The genetic structure of coastal giant salamanders (Dicamptodon tenebrosus) in a managed forest
The genetic structure of coastal giant salamanders (Dicamptodon tenebrosus) in a managed forest
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DOI：
10.1016/s0006-3207(03)00092-2
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发表时间：
2004-01-01
影响因子：
5.9
通讯作者：
Taylor, EB
中科院分区：
文献类型：
--
作者：
Curtis, JMR;Taylor, EB
火蜥蜴，Dicamptodon tenebrosus，受到与森林采伐活动相关的栖息地丧失和破碎化的威胁。我们使用了3个微卫星位点和38个扩增片段长度多态性（AFLP），以量化人口结构和间接评估森林采伐对这个物种的影响。在加拿大的不列颠哥伦比亚省，我们取样了两个老的生长点，三个第二生长点和三个最近的皆伐点。微卫星等位基因丰富度和多态性AFLP所占比例与林分年龄呈正相关。同样，从两个标记类型估计的杂合性与林分年龄呈正相关。利用微卫星和AFLP标记估计的森林样地的种群细分（F-st）分别为0.033和0.095。较低的遗传变异和杂合性在最近的皆伐表明，皆伐采伐可能与当地人口下降。我们的遗传发现与以前的研究一致，这些研究报告了最近被砍伐的地点的蝾螈密度较低。(C)2003爱思唯尔有限公司。保留所有权利。
The salamander, Dicamptodon tenebrosus, is threatened by habitat loss and fragmentation associated with forest harvest activities. We used three microsatellite loci and 38 amplified fragment length polymorphisms (AFLPs) to quantify population structure and indirectly evaluate the impacts of forest harvesting on this species. We sampled two old growth sites, three second growth sites and three recently clearcut sites in British Columbia, Canada. Microsatellite allelic richness and the percentage of polymorphic AFLPs were positively correlated with the age of forest stands. Similarly, heterozygosity estimated from both marker types was positively correlated with stand age. Population subdivision (F-st) estimated among forested sites using microsatellite and AFLP markers was 0.033 and 0.095, respectively. Lower genetic variation and heterozygosity in recent clearcuts suggest that clearcut logging may be associated with local population declines. Our genetic findings are consistent with previous studies that report lower salamander densities in recently clearcut sites. (C) 2003 Elsevier Ltd. All rights reserved.