Identification of a mitochondrial glycerol-3-phosphate dehydrogenase from Arabidopsis thaliana:: evidence for a mitochondrial glycerol-3-phosphate shuttle in plants
Identification of a mitochondrial glycerol-3-phosphate dehydrogenase from Arabidopsis thaliana:: evidence for a mitochondrial glycerol-3-phosphate shuttle in plants
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DOI：
10.1016/s0014-5793(03)00033-4
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发表时间：
2003-02-11
期刊：
影响因子：
3.5
通讯作者：
Zou, JT
中科院分区：
文献类型：
--
作者：
Shen, WY;Wei, YD;Zou, JT
我们报道了一种拟南芥基因的分子特征，该基因编码线粒体fad依赖的甘油-3-磷酸脱氢酶（FAD-GPDH），该酶将甘油-3-磷酸（G-3-P）氧化为磷酸二羟基丙酮。我们通过体外靶向实验证明，编码的基因产物可以导入线粒体膜系统。通过大肠杆菌的外源表达证实了该蛋白的酶活性。拟南芥基因在植物发育过程中表达，但在种子萌发时表达水平最高。我们还发现，拟南芥FAD-GPDH基因的表达与氧消耗有关，并受ABA和胁迫条件的影响。与NAD+依赖的GPDH一起，该酶可以形成G-3-P穿梭体，如先前在其他真核生物中建立的那样，并将细胞质G-3-P代谢与植物的碳源利用和能量代谢联系起来。(C) 2003年由Elsevier Science B.V.代表欧洲生化学会联合会出版。
We report molecular characterization of an Arabidopsis gene encoding a mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (FAD-GPDH) that oxidizes glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate. We demonstrate through in vitro targeting assays that the encoded gene product can be imported into mitochondrial membrane systems. Enzyme activity of the protein was confirmed through heterologous expression in Escherichia coli. The Arabidopsis gene is expressed throughout plant development, but at the highest level during seed germination. We also show that expression of the Arabidopsis FAD-GPDH gene is coupled to oxygen consumption and affected by ABA and stress conditions. Together with an NAD+-dependent GPDH, this enzyme could form a G-3-P shuttle, as previously established in other eukaryotic organisms, and links cytosolic G-3-P metabolism to carbon source utilization and energy metabolism in plants. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.