Novel ELISA system for detection of N-ERC/mesothelin in the sera of mesothelioma patients
Novel ELISA system for detection of N-ERC/mesothelin in the sera of mesothelioma patients
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DOI：
10.1111/j.1349-7006.2006.00246.x
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发表时间：
2006-09-01
期刊：
影响因子：
5.7
通讯作者：
Hino, Okio
中科院分区：
文献类型：
--
作者：
Shiomi, Kazu;Miyamoto, Hideaki;Hino, Okio
我们已经开发了一种新的酶联免疫吸附测定（ELISA）系统检测间皮瘤患者血清中的N-ERC/间皮素，并已开始研究其临床实用性。N-ERC/间皮素是形成全长71-kDa ERC/间皮素蛋白的N-末端片段的31-kDa蛋白，并且生理性地分泌到间皮瘤患者的血液中，在那里可以使用我们的含有两种抗体（兔多克隆抗ERC/间皮素抗体-282和小鼠单克隆抗体7 E7）的夹心ELISA来检测它。迄今为止，我们的ELISA系统在间皮瘤患者中检测到的N-ERC/间皮素的血清水平比健康对照或患有其他肺或胸膜疾病的患者高得多。总之，N-ERC/间皮素是间皮瘤的有希望的候选肿瘤标志物。
We have developed a novel enzyme-linked immunosorbent assay (ELISA) system for the detection of N-ERC/mesothelin in the serum of mesothelioma patients and have begun to examine its clinical usefulness. N-ERC/mesothelin is a 31-kDa protein that forms the N-terminal fragment of the full-length 71-kDa ERC/mesothelin protein, and is physiologically secreted into the blood of mesothelioma patients where it can be detected using our sandwich ELISA containing two antibodies (rabbit polyclonal anti-ERC/mesothelin antibody-282 and mouse monoclonal antibody 7E7). Our ELISA system has thus far detected much higher serum levels of N-ERC/mesothelin in mesothelioma patients than in healthy controls or patients with other lung or pleural diseases. In conclusion, N-ERC/mesothelin is a promising candidate tumor marker for mesothelioma.