Mitochondrial DNA Transport in Drosophila Neurons.
Mitochondrial DNA Transport in Drosophila Neurons.
复制标题
果蝇神经元中的线粒体 DNA 运输。
DOI：
10.1007/978-1-0716-1990-2_21
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Bateman JM
中科院分区：
文献类型：
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作者：
Bateman JM
线粒体是产生能量并在细胞代谢中发挥重要作用的重要细胞器。小圆形线粒体基因组编码线粒体呼吸器的关键组分。线粒体DNA（mtDNA）的缺失或突变导致线粒体功能障碍和疾病。线粒体DNA被包装成类核，类核在线粒体内被运输到整个细胞。类核的有效运输在神经元中是必不可少的，其中线粒体功能在突触处局部需要。在这里，我描述的方法可视化的拟核inDrosophilopterions使用GFP融合的线粒体转录因子TFAM。TFAM-GFP与mCherry标记的线粒体一起用于使固定的幼虫节段神经中的类核可视化。我还描述了如何这些工具可以用于实时成像的类核动力学。使用果蝇作为模型系统，这些方法将能够进一步表征和分析神经元中的类核动力学。
Mitochondria are essential organelles that generate energy and play vital roles in cellular metabolism. The small circular mitochondrial genome encodes key components of the mitochondrial respiratory apparatus. Depletion of, or mutations in mitochondrial DNA (mtDNA) cause mitochondrial dysfunction and disease. mtDNA is packaged into nucleoids, which are transported throughout the cell within mitochondria. Efficient transport of nucleoids is essential in neurons, where mitochondrial function is required locally at synapses. Here I describe methods for visualization of nucleoids inDrosophilaneurons using a GFP fusion of the mitochondrial transcription factor TFAM. TFAM-GFP, together with mCherry-labeled mitochondria, was used to visualize nucleoids in fixed larval segmental nerves. I also describe how these tools can be used for live imaging of nucleoid dynamics. UsingDrosophilaas a model system, these methods will enable further characterization and analysis of nucleoid dynamics in neurons.