Isolation and Identification of the DNA Aptamer Target to Acetamiprid
Isolation and Identification of the DNA Aptamer Target to Acetamiprid
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DOI：
10.1021/jf104189g
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发表时间：
2011-03-09
影响因子：
6.1
通讯作者：
Liu, Xianjin
中科院分区：
文献类型：
--
作者：
He, Jiang;Liu, Yuan;Liu, Xianjin
作为抗体的替代物，适体作为农药检测的分析工具具有很大的潜力。在这项工作中，通过指数富集配体的特定系统进化（SELEX）策略，其中单链DNA（ssDNA）文库被固定化，并消除靶标修饰，选择了靶向啶虫脒的适体。经过18轮重复选择，富集ssDNA池，然后选择14个序列并仔细鉴定。最后，成功地获得了啶虫脒特异性适配子，其表观解离常数（K-d）估计为4.98 μ M。目前正在开展进一步的工作，以开发一种基于适体的检测方法，用于农产品和环境样品中这种农药的现场测定。
As an alternative to antibodies, aptamers have a great potential as analytical tools for pesticide detection. In this work, aptamers targeting acetamiprid were selected by a specific systematic evolution of ligands by exponential enrichment (SELEX) strategy, where a single-stranded DNA (ssDNA) library was immobilized and target modification was eliminated. After 18 rounds of repeated selection, the ssDNA pool was enriched and then 14 sequences were selected and carefully identified. At last, an acetamiprid-specific aptamer with the apparent dissociation constant (K-d) estimated to be 4.98 mu M was successfully obtained. Further work is ongoing to develop an aptamer-based detection method for field determination of this pesticides in agricultural products and environmental samples.