A phospholipase A2 isoenzyme provokes lipoxin B formation from endogenous sources of arachidonic acid in porcine leukocytes.
A phospholipase A2 isoenzyme provokes lipoxin B formation from endogenous sources of arachidonic acid in porcine leukocytes.
复制标题
磷脂酶 A2 同工酶可激发猪白细胞内源性花生四烯酸形成脂氧素 B。
DOI：
10.1016/s0006-291x(87)80484-9
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发表时间：
1987
影响因子：
3.1
通讯作者：
Wong,PY
中科院分区：
文献类型：
--
作者：
Lam,BK;Serhan,CN;Samuelsson,B;Wong,PY
用磷脂酶A2（PLA 2）（从蛇毒中分离）的同工酶孵育猪白细胞，产生几种含三羟基四烯的化合物，这些化合物来源于花生四烯酸的内源性来源。这些化合物的形成具有剂量依赖性，EC 50约为1.25 × 10− 8 M。在该同工酶浓度和暴露时间下，通过排除台盼蓝测定细胞保持活力。通过HPLC纯化化合物，并通过包括紫外光谱法、GC/MS的物理标准以及通过与合成和真实材料的比较来确定它们的身份。生物衍生化合物被证明是脂氧素B（5S，14 R，15 S-三羟基-6，10，12-反式-8-二十碳四烯酸）及其两种结构异构体（8-trans-LX B和14 S-8-trans-LX B）。有趣的是，在这些孵育中仅发现少量脂氧素A及其异构体。本研究结果表明，猪白细胞可以从内源性花生四烯酸来源产生脂氧素B及其异构体。此外，他们认为某些PLA 2同工酶可能启动脂氧素和相关化合物的形成。
Porcine leukocytes incubated with an isoenzyme of phospholipase A2(PLA2) (isolated from snake venom) produced several trihydroxytetraene- containing compounds which were derived from endogenous sources of arachidonic acid. The formation of these compounds was dose-dependent with an EC50of approximately 1.25 × 10−8M. At this concentration of the isoenzyme and time of explosure the cells remained viable as determined by the exclusion of trypan blue. The compounds were purified by HPLC and their identities were determined by physical criteria which included U.V. spectrometry, GC/MS and by comparison with both synthetic and authentic materials. The biologically derived compounds proved to be lipoxin B (5S, 14R, 15S-trihydroxy-6, 10, 12-trans-8-ciseicosatetraenoic acid) and its two structural isomers (8-trans-LXB and 14S-8-trans-LXB). Of interest, only small amounts of lipoxin A and its isomers were found in these incubations. Results of the present study indicate that porcine leukocytes can generate lipoxin B and its isomers from endogenous sources of arachidonic acid. Moreover, they suggest that certain PLA2isoenzymes may initiate the formation of lipoxins and related compounds.