Antimicrobial activity of a quaternary ammonium methacryloxy silicate-containing acrylic resin: a randomised clinical trial.
Antimicrobial activity of a quaternary ammonium methacryloxy silicate-containing acrylic resin: a randomised clinical trial.
复制标题
DOI：
10.1038/srep21882
复制
发表时间：
2016-02-23
影响因子：
4.6
通讯作者：
Tay FR
中科院分区：
文献类型：
--
作者：
Liu SY;Tonggu L;Niu LN;Gong SQ;Fan B;Wang L;Zhao JH;Huang C;Pashley DH;Tay FR
含甲基丙烯氧基硅酸季铵盐(QAMS)的丙烯酸树脂经3个月的水储存后，在体外表现出较强的抗接触杀菌能力。目前的双盲随机临床试验的目的是通过使用定制的可拆卸固位体来确定含有QAMS的正畸丙烯酸的体内抗菌效果。32名受试者口腔内佩戴这种固位体，使用分口研究设计，创建48小时的多物种菌斑生物膜。将两个不含QAMS的对照亚克力圆盘插入正畸固位体一侧的孔中，将两个实验用的含QAMS的亚克力圆盘插入同一固位体另一侧的孔中。48小时后，取回圆盘，并使用共聚焦激光扫描显微镜检查微生物活力。对口腔粘膜和全身健康均无损害。在没有交叉效应和分配偏差(插入固位体左侧或右侧的盘片)的情况下，从受试者获取的无QAM质控照盘的生物体积杀伤率(3.73 ± 2.11%)与含QAM质控盘的生物体积杀伤率(33.94 ± 23.88%)有显著差异(P ≤ 0.001)。结果表明，含有QAMS的丙烯酸酯在体内对菌斑生物膜具有良好的抗菌活性。含有QAMS的丙烯酸也可用于制造可拆卸的丙烯酸义齿。
Quaternary ammonium methacryloxy silicate (QAMS)-containing acrylic resin demonstrated contact-killing antimicrobial ability in vitro after three months of water storage. The objective of the present double-blind randomised clinical trial was to determine the in vivo antimicrobial efficacy of QAMS-containing orthodontic acrylic by using custom-made removable retainers that were worn intraorally by 32 human subjects to create 48-hour multi-species plaque biofilms, using a split-mouth study design. Two control QAMS-free acrylic disks were inserted into the wells on one side of an orthodontic retainer, and two experimental QAMS-containing acrylic disks were inserted into the wells on the other side of the same retainer. After 48 hours, the disks were retrieved and examined for microbial vitality using confocal laser scanning microscopy. No harm to the oral mucosa or systemic health occurred. In the absence of carry-across effect and allocation bias (disks inserted in the left or right side of retainer), significant difference was identified between the percentage kill in the biovolume of QAMS-free control disks (3.73 ± 2.11%) and QAMS-containing experimental disks (33.94 ± 23.88%) retrieved from the subjects (P ≤ 0.001). The results validated that the QAMS-containing acrylic exhibits favourable antimicrobial activity against plaque biofilms in vivo. The QAMS-containing acrylic may also be used for fabricating removable acrylic dentures.