Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels
Facile method for CLSM imaging unfunctionalized Au nanoparticles through fluorescent channels
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DOI：
10.1007/s11051-009-9618-x
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发表时间：
2009-03
影响因子：
2.5
通讯作者：
Lan Yuan;Wei Wei-Wei;Juan Li;Zhiwei Sun;Hongfang Wang;Xiuzhi Zhang;Yueyue Chen
中科院分区：
文献类型：
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作者：
Lan Yuan;Wei Wei-Wei;Juan Li;Zhiwei Sun;Hongfang Wang;Xiuzhi Zhang;Yueyue Chen
金属纳米粒子的显微可视化已经成为研究其在细胞标记和生物效应中的应用的有用工具。在本研究中，我们发展了一种利用共聚焦激光扫描显微镜（CLSM）通过荧光通道观察非功能化Au纳米颗粒的简便方法。金属纳米颗粒的尖锐反射信号和光稳定性使得本方法对于荧光共定位、实时成像和进一步的定量分析非常理想。
The microscopic visualization of metal nanoparticles has become a useful tool for the investigation of their applications in cell labeling and the study of their bio-effects. In the current study, we have developed a facile method with confocal laser scanning microscope (CLSM) to observe unfunctionalized Au nanoparticles through fluorescent channels. The sharp reflected signal and photostable property of the metal nanoparticles makes the present method very ideal for fluorescent co-localization, real-time imaging, and further quantitative analysis.