Oral immunization with the B subunit of the heat-labile enterotoxin of Escherichia coli induces early Th1 and late Th2 cytokine expression in Peyer's patches.
Oral immunization with the B subunit of the heat-labile enterotoxin of Escherichia coli induces early Th1 and late Th2 cytokine expression in Peyer's patches.
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DOI：
10.1093/infdis/173.6.1428
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发表时间：
1996-06
期刊：
影响因子：
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通讯作者：
Ichiro Nakagawa;I. Takahashi;Hiroshi Kiyono;Jerry R. McGhee;Shigeyuki Hamada
中科院分区：
文献类型：
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作者：
Ichiro Nakagawa;I. Takahashi;Hiroshi Kiyono;Jerry R. McGhee;Shigeyuki Hamada
来自产肠毒素大肠杆菌的热不稳定毒素（LT-B）的B亚基已被证明是一种强大的粘膜免疫原。口服免疫lb - b小鼠显示，BALB/c （H-2d）和C57BL/6 （H-2b）小鼠对lb - b具有较高的血清IgG和黏膜IgA特异性应答。然而，缺乏肠道Peyer’s patches （PP）的ALY （H-2b）小鼠对口服LT-B没有血清或粘膜抗体反应。这些结果表明，当口服抗原时，PP淋巴细胞支持全身和粘膜免疫反应。逆转录聚合酶链反应分析显示，PP CD4 T细胞表达早期th1型（干扰素- γ和白细胞介素[IL]-2）和晚期th2型（IL-4， -5和-6）细胞因子mRNA。这些结果表明，LT-B诱导的全身和粘膜抗体反应是由PP Th1和Th2细胞共同调节的。
The B subunit of heat-labile toxin (LT-B) from enterotoxigenic Escherichia coli has been shown to be a powerful mucosal immunogen. Oral immunization of mice with LT-B revealed that BALB/c (H-2d) and C57BL/6 (H-2b) mice gave high serum IgG and mucosal IgA responses specific for LT-B. However, ALY (H-2b) mice lacking intestinal Peyer's patches (PP) did not respond to oral LT-B with either serum or mucosal antibodies. These results indicate that PP lymphocytes supported both systemic and mucosal immune responses when the antigen was administered orally. Reverse transcription polymerase chain reaction analyses revealed that PP CD4 T cells expressed early Th1-type (interferon-gamma and interleukin [IL]-2) and late Th2-type (IL-4, -5, and -6) cytokine mRNA. These results suggest that the systemic and mucosal antibody responses induced by LT-B are regulated by a cooperation of PP Th1 and Th2 cells.