Focusing proteins in an electric field gradient
Focusing proteins in an electric field gradient
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DOI：
10.1016/0021-9673(95)01069-6
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发表时间：
1996-03-01
影响因子：
4.1
通讯作者：
Ivory, CF
中科院分区：
文献类型：
--
作者：
Koegler, WS;Ivory, CF
梯度技术是将分离和浓缩步骤联合收割机结合起来的一类分离方法。这类技术使用一个或多个梯度的反作用力来创建一个蛋白质可以集中的“平衡点”。平衡梯度技术可以通过基于蛋白质的物理特性的差异选择适当的反作用力来适应特定的分离。这里介绍的方法，场梯度聚焦，是这类分离技术的补充，它采用电场梯度来同时分离和浓缩分子。
Gradient techniques are a class of separation methods that combine the steps of isolation and concentration. This class of techniques uses one or more gradients of counteracting forces to create an 'equilibrium point' at which a protein can focus. Equilibrium gradient techniques can be adapted to a specific separation by choosing appropriate counteracting forces based on differences in the physical characteristics of proteins. The method introduced here, field gradient focusing, is an addition to this class of separation techniques which employs a gradient in the electric field to simultaneously separate and concentrate molecules.