Characterization of the inhibitory effects of retinoids on the in vitro growth of two malignant murine melanomas.
Characterization of the inhibitory effects of retinoids on the in vitro growth of two malignant murine melanomas.
复制标题
类维生素A对两种恶性小鼠黑色素瘤体外生长的抑制作用的表征。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
G. Nicolson
中科院分区：
文献类型：
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作者：
R. Lotan;G. Giotta;E. Nork;G. Nicolson
维甲酸和维甲酸乙酯对小鼠黑色素瘤细胞株S91和B16的体外增殖均有抑制作用。对S91和B16黑色素瘤细胞的抑制率分别由10(-9)M视黄酸的55%和30%增加到85%和82%。S91黑色素瘤细胞对维甲酸的抑制作用比B16黑色素瘤细胞更敏感，且两种细胞系对维甲酸的敏感性均高于对维甲酸乙酯的敏感性。当暴露于10(-5)M维甲酸时，这两种类型的细胞在48小时内以与对照细胞相同的速度生长，因此维甲酸处理的细胞的生长速度下降。培养6天后，对照组细胞数增加140倍(S91黑色素瘤细胞)和265倍(B16黑色素瘤细胞)，而维甲酸处理细胞仅增加14倍(S91黑色素瘤细胞)和40倍(B16黑色素瘤细胞)。维甲酸对细胞生长的抑制程度与初始细胞密度无关。可的松和氢化可的松不能阻止或减弱维甲酸的抑制作用，10(-5)M维甲酸作用5d的细胞，溶酶体酸性磷酸酶的释放不增加，细胞内3‘，5’-环磷酸腺苷水平不升高。S91和B16细胞在10(-5)M维甲酸作用8天后的存活率与对照组相似。从培养上清液中去除维甲酸后48-72小时，维甲酸处理的细胞生长速度下降，恢复到对照水平。
The in vitro proliferation of murine melanoma cell lines S91 and B16 was inhibited by retinoic acid and retinyl acetate. The inhibitory effects were dependent on retinoid concentration and increased from 55 and 30% at 10(-9) M retinoic acid to 85 and 82% at 10(-5) M retinoic acid for S91 and B16 melanoma cells, respectively. S91 melanoma cells were more sensitive than B16 melanoma cells to inhibition by either retinoid, and both cell lines were more sensitive to retinoic acid than to retinyl acetate. When exposed to 10(-5) M retinoic acid, the two cell types grew at the same rate as did control cells for 48 hours, whereupon the growth rates of retinoid-treated cells decreased. After 6 days, the number of cells in control cultures increased 140 times (S91 melanoma cells) and 265 times (B16 melanoma cells), whereas retinoic acid-treated cells increased only 14 times (S91 melanoma cells) and 40 times (B16 melanoma cells). The degree of growth inhibition by retinoic acid was not dependent on initial cell density. Cortisone and hydrocortisone failed to prevent or reduce the inhibitory effect of retinoic acid; the release of lysosomal acid phosphatase was not increased and the intracellular level of 3',5'-cyclic AMP in cells grown for 5 days in the presence of 10(-5) M retinoic acid was not elevated. Viability of S91 and B16 cells after 8 days' exposure to 10(-5) M retinoic acid was similar to that in control cultures. The reduced growth rate of retinoic acid-treated cells reversed to the control rate 48-72 hours after removal of retinoic acid from the growth medium.