Light Signaling-Dependent Regulation of PSII Biogenesis and Functional Maintenance1[OPEN]
Light Signaling-Dependent Regulation of PSII Biogenesis and Functional Maintenance1[OPEN]
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PSII 生物发生和功能维持的光信号依赖性调节(1)([OPEN])
DOI：
10.1104/pp.20.00200
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发表时间：
2020-08-01
期刊：
影响因子：
7.4
通讯作者：
Jin, Hong-Lei
中科院分区：
文献类型：
--
作者：
Li, Xue;Wang, Hong-Bin;Jin, Hong-Lei
光是调节植物光形态建成和光合作用的重要环境因子。然而，光信号通路与光系统功能之间相互作用的分子机制尚不清楚。在这里，我们表明，各种单色波长的光合作，以调节PSII功能在拟南芥（拟南芥）。光感受器隐花色素和光敏色素调节高叶绿素荧光173（HCF 173）的表达，HCF 173是PSII生物发生所需的，通过调节由转录因子伸长下胚轴5（HY 5）介导的PSII核心蛋白D1的合成。HY 5直接与HCF 173启动子中的ACGT元件ACE基序和G-box顺式元件结合并调节其活性。5个突变体在不同单色光波长下PSII活性均显著降低。有趣的是，我们证明HY 5还直接调节与PSII组装和修复相关的基因的表达，包括ALBINO 3，HCF 136，对强光敏感1等，这是光损伤条件下PSII功能维持所需的。此外，HY 5的缺乏广泛地减少了除PSII蛋白外的其他光系统蛋白的积累。因此，我们的研究揭示了光信号在光系统的生物发生和功能调节中的重要作用，并提供了深入了解光信号和光合作用之间的联系在陆地plants. Photoceptor光敏色素和隐花色素调节PSII的生物合成，组装和修复的基因表达的调节介导的伸长下胚轴5（HY 5）。
Light is a key environmental cue regulating photomorphogenesis and photosynthesis in plants. However, the molecular mechanisms underlying the interaction between light signaling pathways and photosystem function are unknown. Here, we show that various monochromatic wavelengths of light cooperate to regulate PSII function in Arabidopsis (Arabidopsis thaliana). The photoreceptors cryptochromes and phytochromes modulate the expression of HIGH CHLOROPHYLL FLUORESCENCE173 (HCF173), which is required for PSII biogenesis by regulating PSII core protein D1 synthesis mediated by the transcription factor ELONGATED HYPOCOTYL5 (HY5). HY5 directly binds to the ACGT-containing element ACE motif andG-boxcis-element present in theHCF173promoter and regulates its activity. PSII activity was decreased significantly inhy5mutants under various monochromatic wavelengths of light. Interestingly, we demonstrate that HY5 also directly regulates the expression of the genes associated with PSII assembly and repair, includingALBINO3,HCF136,HYPERSENSITIVE TO HIGH LIGHT1, etc., which is required for the functional maintenance of PSII under photodamaging conditions. Moreover, deficiency ofHY5broadly decreases the accumulation of other photosystem proteins besides PSII proteins. Thus, our study reveals an important role of light signaling in both biogenesis and functional regulation of the photosystem and provides insight into the link between light signaling and photosynthesis in land plants.The photoreceptors phytochrome and cryptochrome regulate PSII biogenesis, assembly, and repair mediated by regulation of gene expression by ELONGATED HYPOCOTYL5 (HY5).