Immunoelectron microscopic study for polyamines
Immunoelectron microscopic study for polyamines
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DOI：
10.1177/002215549804601112
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发表时间：
1998-11-01
影响因子：
3.2
通讯作者：
Tsuru, D
中科院分区：
文献类型：
--
作者：
Fujiwara, K;Bai, G;Tsuru, D
多胺（PAs）是真核和原核细胞中普遍存在的多聚阳离子代谢产物，被认为与DNA、RNA和蛋白质生物合成的调控密切相关，但其确切功能尚不清楚，主要是由于缺乏对PA亚细胞定位的认识。在这项研究中，使用免疫电镜，我们已经证明，PA主要位于大鼠延髓外侧网状核的神经元的粗面内质网的游离和附着的核糖体。核、轴突和神经末梢均无PA。这表明多胺是具有生物活性的核糖体的组成部分之一，与蛋白质生物合成的翻译过程密切相关。
The polyamines (PAs) are ubiquitous polycationic metabolites in eukaryotic and prokaryotic cells and are believed to be intimately involved in the regulation of DNA, RNA, and protein biosynthesis, the exact function of which remains unclear, mainly because of a lack of knowledge of PA subcellular localization. In this study, using immunoelectron microscopy, we have demonstrated that PAs are predominantly located on free and attached ribosomes of the rough endoplasmic reticulum in the neurons of the lateral reticular nucleus of rat medulla oblongata. The nuclei, axons, and nerve endings were devoid of PA. This suggests that PAs are one of the components of biologically active ribosomes, being closely involved in the translation processes of protein biosynthesis.