Separating changes in the intra- and extracellular water apparent diffusion coefficient following focal cerebral ischemia in the rat brain
Separating changes in the intra- and extracellular water apparent diffusion coefficient following focal cerebral ischemia in the rat brain
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DOI：
10.1002/mrm.10296
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发表时间：
2002-11-01
影响因子：
3.3
通讯作者：
Sotak, CH
中科院分区：
文献类型：
--
作者：
Silva, MD;Omae, T;Sotak, CH
选择性测定了正常和缺血大鼠脑内（IC）和细胞外（EC）脑水表观弥散系数（ADC）值。选择性T-1-松弛增强EC水，使用脑室内（ICV）输液的NMR造影剂（CR），被用来分离IC和EC信号的贡献。在CR灌注的正常脑（n = 4）中，IC水（85%）的T-1 = 235 +/-10 ms和T-2 = 46 +/-2 ms，EC水（15%）的T-1 = 48 +/-8 ms和T-2 = 6 +/-2 ms。体积局部ADC（z）正常、CR输注、缺血和缺血/CR输注组的（z梯度轴）值分别为0.90 +/-0.02（EC + IC）、0.81 +/-0.05（IC）、0.51 +/-0.02（EC + IC）和0.53 +/-0.07（IC（ADC值为x 10（-3）mm（2）/s;每组n = 5）。同一组的成像ADC（z）值分别为0.81 +/-0.03（EC + IC）、0.75 +/-0.05（IC）、0.51 +/-0.04（EC + IC）和0.52 +/-0.05（IC）。同一组的成像ADC（av）（平均扩散率）值分别为0.70 +/-0.05（EC + IC）、0.69 +/-0.06（IC）、0.45 +/-0.06（EC + IC）和0.44 +/-0.06（IC）。这些结果表明，IC水ADC决定了正常和缺血大鼠脑的总体水ADC值。(C)2002 Wiley-Liss，Inc.
Selective intracellular (IC) and extracellular (EC) brain water apparent diffusion coefficient (ADC) values were measured in normal and ischemic rat brain. Selective T-1-relaxation enhancement of the EC water, using intracerebroventricular (ICV) infusion of an NMR contrast reagent (CR), was used to separate the IC and EC signal contributions. In the CR-infused, normal brain (n = 4), T-1 = 235 +/- 10 ms and T-2 = 46 +/- 2 ms for IC water (85%) and T-1 = 48 +/- 8 ms and T-2 = 6 +/- 2 ms for EC water (15%). Volume-localized ADC(z) (z-gradient axis) values were 0.90 +/- 0.02 (EC+IC), 0.81 +/- 0.05 (IC), 0.51 +/- 0.02 (EC+IC), and 0.53 +/- 0.07 (IC), for normal, CR-infused, ischemic, and ischemic/CR-infused groups, respectively (ADC values are x 10(-3) mm(2)/s; n = 5 for each group). Imaging ADC(z) values were 0.81 +/- 0.03 (EC+IC), 0.75 +/- 0.05 (IC), 0.51 +/- 0.04 (EC+IC), and 0.52 +/- 0.05 (IC), respectively, for the same groups. Imaging ADC(av) (average diffusivity) values for the same groups were 0.70 +/- 0.05 (EC+IC), 0.69 +/- 0.06 (IC), 0.45 +/- 0.06 (EC+IC), and 0.44 +/- 0.06 [IC), respectively. These results suggest that the IC water ADC determines the overall water ADC value in normal and ischemic rat brain. (C) 2002 Wiley-Liss, Inc.