Identification and quantification of tetrodotoxin in the marine gastropod Nassarius by LC-MS
Identification and quantification of tetrodotoxin in the marine gastropod Nassarius by LC-MS
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DOI：
10.1016/j.toxicon.2007.12.003
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发表时间：
2008-04-01
期刊：
影响因子：
2.8
通讯作者：
Lin, Hong-Lin
中科院分区：
文献类型：
--
作者：
Huang, Hong-Nan;Lin, Jie;Lin, Hong-Lin
海洋及其他来源中河豚毒素的分析方法通常采用高效液相色谱分离、碱处理和荧光分析。然而，这些方法的应用受到限制，因为他们繁琐的提取和推导过程。本文建立了一种简单、快速的液相色谱-质谱联用方法，并应用该方法对采自中国斐济的织纹螺属海产腹足类中的河豚毒素进行了定量分析。通过NIS和MSn证实该毒素与真实TTX相当。此外，通过这种新方法在织纹螺中检测到中等量的TTX，表明织纹螺可能是一种潜在的食物中毒源。(c)2007爱思唯尔有限公司保留所有权利。
Tetrodotoxin (TTX) has been usually analyzed in marine and other sources by HPLC separation, followed by alkaline treatment and fluorescence assay. However, the applications of such methods were limited because of their tedious extraction and derivation procedures. A simpler, rapid LC-MS method, using selected ion-monitoring, has now been developed, and used to identify and quantitate TTX in a marine gastropod of the genus Nassarius, collected from Fijian province of China. The toxin was confirmed comparable to authentic TTX by NIS and MSn. In addition, a moderate amount of TTX was detected in the Nassarius by this novel approach, suggesting that Nassarius would be a potential food poison source. (c) 2007 Elsevier Ltd. All rights reserved.