Comparative analysis of the Geobacillus hemicellulose utilization locus reveals a highly variable target for improved hemicellulolysis.
Comparative analysis of the Geobacillus hemicellulose utilization locus reveals a highly variable target for improved hemicellulolysis.
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DOI：
10.1186/1471-2164-15-836
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发表时间：
2014-10-01
期刊：
影响因子：
4.4
通讯作者：
Cowan DA
中科院分区：
文献类型：
--
作者：
De Maayer P;Brumm PJ;Mead DA;Cowan DA
嗜热土芽孢杆菌属的成员可以在高温下生长，并产生一系列热稳定的半纤维素水解酶，使其成为生物质生物转化为增值产品的理想候选者。迄今为止，半纤维素降解和利用的分子决定因素仅在一种菌株中被鉴定和部分表征，即嗜热脂肪土芽孢杆菌T-6，其中它们聚集在单个遗传位点中。使用G.以嗜热脂肪菌（stearothermophilus）T-6半纤维素利用位点为遗传标记，在17/24株土芽孢杆菌的全基因组和部分基因组中鉴定出邻位半纤维素利用（HUS）位点。这些HUS基因座位于共同的基因组岛上。这些基因座的比较分析揭示了广泛的变异性之间的土芽孢杆菌半纤维素利用系统，只有7个的41-68个蛋白质编码的这些基因座之间保守的HUS+菌株。这转化为广泛的差异，水解酶，运输系统和代谢途径所采用的土芽孢杆菌属。以降解和利用半纤维素聚合物。土芽孢杆菌HUS基因座之间的遗传变异性意味着它们具有可变的降解半纤维素聚合物的能力，或者它们可以降解不同的聚合物，如在不同的植物物种和组织中发现的。本研究的数据可以作为具有改进的降解和利用半纤维素的能力的土芽孢杆菌菌株的基因工程的基础。本文的在线版本（doi：10.1186/1471-2164-15-836）包含补充材料，可供授权用户使用。
Members of the thermophilic genus Geobacillus can grow at high temperatures and produce a battery of thermostable hemicellulose hydrolytic enzymes, making them ideal candidates for the bioconversion of biomass to value-added products. To date the molecular determinants for hemicellulose degradation and utilization have only been identified and partially characterized in one strain, namely Geobacillus stearothermophilus T-6, where they are clustered in a single genetic locus. Using the G. stearothermophilus T-6 hemicellulose utilization locus as genetic marker, orthologous hemicellulose utilization (HUS) loci were identified in the complete and partial genomes of 17/24 Geobacillus strains. These HUS loci are localized on a common genomic island. Comparative analyses of these loci revealed extensive variability among the Geobacillus hemicellulose utilization systems, with only seven out of 41–68 proteins encoded on these loci conserved among the HUS+ strains. This translates into extensive differences in the hydrolytic enzymes, transport systems and metabolic pathways employed by Geobacillus spp. to degrade and utilize hemicellulose polymers. The genetic variability among the Geobacillus HUS loci implies that they have variable capacities to degrade hemicellulose polymers, or that they may degrade distinct polymers, as are found in different plant species and tissues. The data from this study can serve as a basis for the genetic engineering of a Geobacillus strain(s) with an improved capacity to degrade and utilize hemicellulose. The online version of this article (doi:10.1186/1471-2164-15-836) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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