Survival of mature CD4 T lymphocytes is dependent on major histocompatibility complex class II-expressing dendritic cells.
Survival of mature CD4 T lymphocytes is dependent on major histocompatibility complex class II-expressing dendritic cells.
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DOI：
10.1084/jem.186.8.1223
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发表时间：
1997-10-20
期刊：
影响因子：
--
通讯作者：
Brocker T
中科院分区：
文献类型：
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作者：
Brocker T
胸腺T细胞发育受T细胞受体（TCR）-主要组织相容性复合体（MHC）相互作用的控制，而外周成熟T细胞对TCR-MHC接触的进一步依赖性迄今尚未被描述。为了研究这个问题，在缺乏MHC II类表达的小鼠和仅在树突状细胞上表达MHC II类的小鼠中调查了CD 4 T细胞存活。由于这些小鼠都没有在胸腺中阳性选择CD 4 T细胞，因此将它们与MHC II类阳性胚胎胸腺组织移植，该组织已经耗尽了骨髓来源的细胞。虽然在两个主机的胸腺移植物与宿主来源的胸腺细胞相同的表型和数量，在外周淋巴器官中的积累的CD 4 + T细胞只能观察到在树突状细胞表达MHC II类的小鼠，但不是在小鼠MHC II类完全缺乏。通过组织学评估，发现积聚的外周CD 4 T细胞与MHC II类+树突状细胞密切接触，表明CD 4 T细胞需要外周MHC II类表达才能存活，并且II类+树突状细胞可能对CD 4 T细胞的寿命起重要作用。
Thymic T cell development is controlled by T cell receptor (TCR)–major histocompatibility complex (MHC) interactions, whereas a further dependence of peripheral mature T cells on TCR–MHC contact has not been described so far. To study this question, CD4 T cell survival was surveyed in mice lacking MHC class II expression and in mice expressing MHC class II exclusively on dendritic cells. Since neither of these mice positively select CD4 T cells in the thymus, they were grafted with MHC class II–positive embryonic thymic tissue, which had been depleted of bone marrow derived cells. Although the thymus grafts in both hosts were repopulated with host origin thymocytes of identical phenotype and numbers, an accumulation of CD4+ T cells in peripheral lymphoid organs could only be observed in mice expressing MHC class II on dendritic cells, but not in mice that were completely MHC class II deficient. As assessed by histology, the accumulating peripheral CD4 T cells were found to be in close contact with MHC class II+ dendritic cells, suggesting that CD4 T cells need peripheral MHC class II expression for survival and that class II+ dendritic cells might play an important role for the longevity of CD4 T cells.