ABNORMAL LUNG DEVELOPMENT AND CLEFT-PALATE IN MICE LACKING TGF-BETA-3 INDICATES DEFECTS OF EPITHELIAL-MESENCHYMAL INTERACTION
ABNORMAL LUNG DEVELOPMENT AND CLEFT-PALATE IN MICE LACKING TGF-BETA-3 INDICATES DEFECTS OF EPITHELIAL-MESENCHYMAL INTERACTION
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DOI：
10.1038/ng1295-415
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发表时间：
1995-12-01
期刊：
影响因子：
30.8
通讯作者：
GROFFEN, J
中科院分区：
文献类型：
--
作者：
KAARTINEN, V;VONCKEN, JW;GROFFEN, J
转化生长因子 -β3（TGF -β3）被认为具有广泛的生物学活性。为了研究TGF -β3在发育过程中的功能，通过基因打靶技术制备了TGF -β3基因缺失突变小鼠。纯合的TGF -β3 -/-小鼠在出生后20小时内死亡，具有独特且一致的表型特征，包括肺部发育迟缓以及腭裂形成缺陷。与其他腭裂的基因缺失突变体不同，TGF -β3 -/-小鼠没有其他伴随的颅面异常。这项研究表明TGF -β3在腭和肺的正常形态发生中具有重要作用，并直接表明这种细胞因子参与上皮 - 间质相互作用机制。
A broad spectrum of biological activities has been proposed for transforming growth factor-beta 3 (TGF-beta 3). To study TGF-beta 3 function in development, TGF-beta 3 null mutant mice were generated by gene-targeting. Within 20 hours of birth, homozygous TGF-beta 3 -/- mice die with unique and consistent phenotypic features including delayed pulmonary development and defective palatogenesis. Unlike other null mutants with cleft palate, TGF-beta 3 -/- mice lack other concomitant craniofacial abnormalities. This study demonstrates an essential function for TGF-beta 3 in the normal morphogenesis of palate and lung, and directly implicates this cytokine in mechanisms of epithelial-mesenchymal interaction.