PHRENIC NERVE STIMULATION PROTECTS AGAINST MECHANICAL VENTILATION-INDUCED DIAPHRAGM DYSFUNCTION IN RATS
PHRENIC NERVE STIMULATION PROTECTS AGAINST MECHANICAL VENTILATION-INDUCED DIAPHRAGM DYSFUNCTION IN RATS
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DOI：
10.1002/mus.23850
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发表时间：
2013-12-01
期刊：
影响因子：
3.4
通讯作者：
Li, Shitong
中科院分区：
文献类型：
--
作者：
Yang, Meirong;Wang, Haitao;Li, Shitong
前言：我们探讨了一种新的应用膈神经刺激(PNS)治疗机械通气(MV)所致的隔膜功能障碍的方法。方法：21只SD大鼠随机分为3组：自主呼吸组、18h控制性MV组和18h控制性MV+三七总皂甙组。实验完成后，检测大鼠横隔膜的收缩性能、胰岛素样生长因子-1(IGF-1)和泛素连接酶的基因表达，以及血清IGF-1水平。结果：与自主呼吸大鼠相比，机械通气大鼠的横隔肌收缩功能明显受损，包括力相关特性和力-频率反应。MV还能抑制IGF-1的表达，并诱导肌环指1mRNA的表达。相反，三七总皂甙可抑制MV诱导的横隔膜基因表达变化，恢复横隔膜功能。结论：三七总皂甙通过拮抗IGF-1和泛素连接酶的表达变化，对MV所致的膈肌功能障碍具有保护作用。肌肉神经48：958-962,2013
Introduction: We investigated a novel application of phrenic nerve stimulation (PNS) in diaphragm dysfunction induced by mechanical ventilation (MV). Methods: Twenty-one Sprague-Dawley rats were assigned randomly to 3 groups: spontaneous breathing, 18-h controlled MV, and 18-h controlled MV with PNS. Upon completion of the experimental protocol, diaphragm contractility, gene expression of insulin-like growth factor-1 (IGF-1) and ubiquitin ligases, and serum IGF-1 levels were analyzed. Results: Compared with the spontaneously breathing rats, impaired diaphragm contractile function, including force-related properties and force-frequency responses, were pronounced with MV. Furthermore, MV suppressed IGF-1 and induced muscle ring finger 1 mRNA expression in the diaphragm. In contrast, PNS counteracted MV-induced gene expression changes in the diaphragm and restored diaphragm function. Conclusions: PNS exerted a protective effect against MV-induced diaphragm dysfunction by counteracting altered expression of IGF-1 and ubiquitin ligase in the diaphragm. Muscle Nerve48: 958-962, 2013