Geldanamycin-Induced Morphological Changes Require Candida albicans Hyphal Growth Regulatory Machinery.
Geldanamycin-Induced Morphological Changes Require Candida albicans Hyphal Growth Regulatory Machinery.
复制标题
格尔德霉素诱导的形态变化需要白色念珠菌菌丝生长调节机制。
DOI：
10.1007/s11046-020-00511-3
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发表时间：
2021
期刊：
影响因子：
5.5
通讯作者：
Cleary,IanA
中科院分区：
文献类型：
--
作者：
Saville,StephenP;Cleary,IanA
在白色念珠菌中，格尔德霉素处理抑制了必需的伴侣蛋白Hsp90，并诱导从酵母到丝状形态的变化，可能是通过阻碍细胞周期的进展和分裂。然而，野生型细胞在格尔德霉素暴露后形成的菌丝在外观上与真正的菌丝完全不同。我们已经观察到，格尔德霉素处理引起的形态学的影响似乎在不同的形态调节缺陷的菌株中有所不同。这些结果表明，通过抑制Hsp90 p诱导的丝状形式，虽然不是真正的菌丝，但需要菌丝诱导机制的一些成分来形成。此外，我们还发现BRG 1是一种已知的菌丝形成调节因子，也是氮饥饿诱导假菌丝和格尔德霉素诱导菌丝形成所必需的。
InCandida albicans, geldanamycin treatment inhibits the essential chaperone Hsp90 and induces a change from yeast to filamentous morphology, likely by impeding cell cycle progression and division. However, filaments formed by wild-type cells upon geldanamycin exposure are quite different in appearance from true hyphae. We have observed that effects on morphology caused by geldanamycin treatment appear to vary in strains with defects in different morphological regulators. These results indicate that the filamentous forms induced by inhibiting Hsp90p, while not true hyphae, nonetheless require some components of the hypha induction machinery for their formation. Furthermore, we have found thatBRG1, a known regulator of hypha formation, is also required for pseudohypha induction in response to nitrogen starvation and for the formation of elongated filaments upon exposure to geldanamycin.