Evolution of the mitochondrial ATPase 6 gene in Drosophila: unusually high level of polymorphism in D. melanogaster.
Evolution of the mitochondrial ATPase 6 gene in Drosophila: unusually high level of polymorphism in D. melanogaster.
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果蝇线粒体 ATP 酶 6 基因的进化：黑腹果蝇中异常高水平的多态性。
DOI：
10.1017/s0016672300031360
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
S. Chigusa
中科院分区：
文献类型：
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作者：
Maki Kaneko;Yoko Satta;E. T. Matsuura;S. Chigusa
我们测定了果蝇（Drosophila）的两个兄弟种的线粒体DNA序列，该序列从细胞色素氧化酶亚基I（COI）基因的3'端延伸到COIII基因的5'端，长度为1990bp。simulans和D.毛里求斯。对该菌的NADH脱氢酶亚基2基因和COI基因的部分序列和序列进行了分析。黑腹果蝇D. yakuba分析表明，部分菌株ATPase 6基因的氨基酸替换率较高。黑腹鱼的数量比其他物种多。该基因在D.黑腹果蝇所有基因的同义替换率相对恒定，表明D.黑腹果蝇氨基酸替换在D. melanogaster位于哺乳动物、酵母和大肠杆菌中不保守的位点。杆菌ATP酶6基因的这些位点可能在D.黑腹，和氨基酸取代可以解释为中性突变和随机遗传漂变。
We have determined 1990 bp mitochondrial DNA sequence which extends from 3' end of the cytochrome oxidase subunit I (COI) gene to 5' end of the COIII gene from two sibling species of Drosophila, D. simulans and D. mauritiana. Analyses of the sequences and part of the NADH dehydrogenase subunit 2 gene and the COI gene together with those from D. melanogaster and D. yakuba revealed that amino-acid substitution rate of the ATPase 6 gene seems to be higher in some strains of D. melanogaster than in the other species. High level of amino-acid polymorphism in this gene was observed in D. melanogaster. Synonymous substitution rate is relatively constant in all the genes examined, suggesting that mutation rate is not higher in the ATPase 6 gene of D. melanogaster. The amino-acid substitutions found specifically in D. melanogaster are at the sites which are not conserved among mammals, yeast and E. coli. These sites of the ATPase 6 gene might lose the selective constraint in D. melanogaster, and the amino-acid substitutions can be explained by neutral mutations and random genetic drift.