Oral and intranasal administration of somatostatin DNA vaccine mediated by attenuated Salmonella Enterica Serovar Typhimurium to promote growth of piglets
Oral and intranasal administration of somatostatin DNA vaccine mediated by attenuated Salmonella Enterica Serovar Typhimurium to promote growth of piglets
复制标题
减毒肠沙门氏菌鼠伤寒沙门氏菌介导的生长抑素DNA疫苗口服和鼻内给药促进仔猪生长
DOI：
10.1017/s1751731111000231
复制
发表时间：
2011-08-01
期刊：
影响因子：
3.6
通讯作者：
Yang, L. G.
中科院分区：
文献类型：
--
作者：
Liu, X. B.;Liang, A. X.;Yang, L. G.
一种鼠伤寒沙门菌减毒株已被用作口服和鼻内遗传免疫的载体。在这里，我们评估一种鼠伤寒沙门氏菌疫苗株的效力。CSO22 （pGM-CSF/SS，质粒粒细胞-巨噬细胞集落刺激因子/生长抑素）表达两个拷贝的SS基因。115头2月龄仔猪口服或鼻内接种CSO22 （pGM-CSF/SS）疫苗株，接种剂量分别为5 × 10（10）菌落形成单位（CFU）、5 × 10(9)和5 × 10(8)。口服免疫时，在免疫仔猪体内检测到特异性抗ss抗体。高剂量免疫组（5 × 10(10) CFU） SS抗体水平极显著高于磷酸盐缓冲盐水免疫组（P < 0.01），高剂量免疫组有40%的动物SS抗体阳性。高剂量组在免疫后4周、8周和12周的体重增重分别比对照组增加20.86%、10.26%和15.30%。鼻内免疫低剂量组的生长在整个试验期间（12周）提高了10.23%。综上所述，重组菌株可诱导抗ss抗体，提高免疫仔猪的生长性能，且口服免疫方案优于鼻内免疫方案。
An attenuated strain of Salmonella typhimurium has been used as a carrier for oral and intranasal genetic immunization. Here, we evaluate the efficacy of a vaccine strain of S. typhimurium. CSO22 (pGM-CSF/SS, plasmid granulocyte-macrophage colony-stimulating factor/somatostatin) expressing two copies of SS genes. A total of 115 piglets, aged 2 months old, were either orally or intranasally immunized against the vaccine strain CSO22 (pGM-CSF/SS) with three dosages (5 X 10(10) colony forming units (CFU), 5 X 10(9) CFU and 5 X 10(8) CFU). For oral immunization, the specific anti-SS antibodies were detected in the immunized piglets. The levels of SS antibodies in the high-dose immunized group (5 X 10(10) CFU) were significantly higher than that in the phosphate buffered saline immunized group (P < 0.01) and 40% of animals were positive in SS antibodies in the high-dose immunized group. Moreover, the weight gain of the high-dose group was increased by 20.86%, 10.26% and 15.30% during 4, 8 and 12 weeks, respectively, after immunization in comparison to the control. For intranasal immunization, the growth of the low-dose group was increased by 10.23% in the whole test period (12 weeks). In conclusion, our results suggest that the recombinant strain could elicit anti-SS antibodies and improve the growth performance of immunized piglets, and that the oral immunization program is better than the intranasal program.