Analysis of gene expression profiles of normal human nasal mucosa and nasal polyp tissues by SAGE
Analysis of gene expression profiles of normal human nasal mucosa and nasal polyp tissues by SAGE
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DOI：
10.1016/j.jaci.2006.02.048
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发表时间：
2006-07-01
影响因子：
14.2
通讯作者：
Kang, Sun Mook
中科院分区：
文献类型：
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作者：
Lee, Jae Yong;Lee, Sang Hag;Kang, Sun Mook
背景：通过对鼻息肉和鼻息肉组织中基因表达谱的系统研究，将有助于鼻息肉疾病标志物的研究和鼻息肉形成的一般认识。目的：通过对鼻息肉组织和鼻息肉组织中基因表达的系列分析，确定鼻息肉组织和鼻息肉组织的基因表达谱。结果：我们在正常鼻黏膜和鼻息肉中分别检测到65,305个标签和55,829个标签，分别代表20,629和17,636个潜在的转录本种类。在多次遇到的独特标签中，92%(正常鼻黏膜)和90%(鼻息肉)与已知基因或表达序列标签匹配，而其余的不匹配任何GenBank序列。因此，在正常鼻黏膜和鼻息肉中分别鉴定出504和539个新的转录本。尽管两个文库中大多数转录本的表达水平相似，但有114个转录本的表达差异有统计学意义(P<；0.05)，即其中65个转录本和49个转录本在正常鼻粘膜和鼻息肉中表达水平较高。结论：这些信息对基础知识以及未来对人类鼻黏膜病理生理状况的研究非常有用，为评估各种鼻病的改变因素提供了线索。
Background: A systemic determination of gene expression profiles in nasal polyp compared with normal nasal mucosa would contribute considerably to the investigation of the disease marker in various rhinopathy and general knowledge on the formation of human nasal polyp.Objective: The aim of this study was to identify the transcriptome of the normal human nasal mucosa and nasal polyp by the serial analysis of gene expression.Methods: mRNA was extracted from normal inferior turbinate mucosa and nasal polyp. Short sequences (tags), each one usually corresponding to a distinct transcript, was isolated and concatemerized into long DNA molecules, which were cloned and sequenced.Results: We detected 65,305 tags for normal nasal mucosa and 55,829 tags for nasal polyp, representing 20,629 and 17,636 potential transcripts species, respectively. Of the unique tags encountered more than once, 92% (normal nasal mucosa) and 90% (nasal polyp) matched known genes or expressed sequence tags, whereas the remainder did not match any GenBank sequences. Therefore, 504 and 539 novel transcripts were identified in normal nasal mucosa and nasal polyp, respectively. Although the expression levels of most transcripts in both libraries were similar, 114 transcripts were differentially expressed at a statistically significant level (P < .05)-that is, 65 and 49 transcripts among them were expressed at a higher level in normal nasal mucosa and nasal polyp, respectively.Conclusion: This information should be very useful for basic knowledge as well as for future studies on pathophysiological conditions of human nasal mucosa, providing some clues to evaluate the altered factors in a variety of rhinopathies.Clinical implications: The results of this study might contribute to general knowledge on the formation of human nasal polyp.