Free-radical-scavenging and antioxidant activities of secondary metabolites from reddened Cv. Annurca apple fruits

Free-radical-scavenging and antioxidant activities of secondary metabolites from reddened Cv. Annurca apple fruits
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DOI:
10.1021/jf052632g
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发表时间:
2006-02-08
影响因子:
6.1
通讯作者:
Piscopo, V
Piscopo, V
中科院分区:
农林科学1区
文献类型:
--
作者:
Cefarelli, G;D'Abrosca, B;Piscopo, V

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从cv中分离出43种次生代谢物并进行了鉴定。Annurca苹果果实,一种种植在意大利南部的苹果品种。这个苹果品种在采摘后经历了典型的变红处理。所有化合物都根据它们的光谱数据进行了表征。通过测定其清除DPPH中心点(2,2′-二苯基-1-吡啶肼基自由基)、H2O2和NO(一氧化氮)的能力,以及抑制亚油酸甲酯共轭二烯过氧化物或硫代巴比妥酸活性物质TBARS的形成能力,来检测化合物的自由基清除和抗氧化活性。
Forty-three secondary metabolites were isolated and characterized from cv. Annurca apple fruit, an apple variety cultivated in the south of Italy. This apple cultivar undergoes a typical reddening treatment after collection. All of the compounds were characterized on the basis of their spectroscopic data. The compounds were tested for their radical-scavenging and antioxidant activities by measuring their capacity to scavenge DPPH center dot (2,2'-diphenyl-1-picrylhydrazyl radical), H2O2, and NO (nitric oxide) and to inhibit the formation of methyl linoleate conjugated diene hydroperoxides or TBARS (thiobarbituric acid reactive species).