The production of congenital malformations using tissue antisera. V. Fluorescent localization of teratogenic antisera in the maternal and fetal tissue of the rat.
The production of congenital malformations using tissue antisera. V. Fluorescent localization of teratogenic antisera in the maternal and fetal tissue of the rat.
复制标题
使用组织抗血清产生先天性畸形。
作者：
V. Slotnick;R. Brent
将致畸性大鼠肾脏和胎盘抗血清注射到妊娠8天的大鼠中，并在2天和5天后处死，以研究这些抗血清在母体器官和胚胎部位的定位。这两种抗血清均特异性定位于肾小球、肾上腺、肝、脾的基底膜和壁卵黄囊膜。在年轻胚胎、滋养层、羊膜和许多其他母体组织中未观察到致畸血清的体内定位。这些研究支持试图解释致畸抗血清作用机制的三个假设中的两个，即1）滋养层、蜕膜或胚外膜（在这种情况下为卵黄囊）功能的干扰和2）母体疾病的产生，然后反映在胎儿中（定位于许多母体器官）。这些研究不支持对胎儿的直接影响，但也没有反驳。
最后，卵黄囊病理可能导致致畸的假设的意义进行了讨论，因为它可能有重要的影响，不仅关于抗血清诱导的畸形，但因为这些发现可能适用于化学品和其他环境影响引起的畸形。
Teratogenic rat kidney and placental antisera were injected into rats that were eight days pregnant and were sacrificed two and five days later to study the localization of these antisera in the maternal organs and embryonic sites. Both these antisera had specific localization in the basement membrane of the kidney glomeruli, adrenal, liver, spleen and parietal yolk sac membrane. No in vivo localization of teratogenic sera was observed in the young embryo, the trophoblast, the amnion and many other maternal tissues. These studies lend support to two of the three hypotheses that attempt to explain the mechanism of action of teratogenic antisera, namely, 1) the interference of the function of the trophoblast, decidua or extraembryonic membranes (in this case, the yolk sac) and 2) production of maternal disease which is then reflected in the fetus (localization in many maternal organs). A direct effect on the fetus was not supported by these studies, but was not refuted by them either.
Finally, the significance of the hypothesis that yolk sac pathology could result in teratogenesis is discussed, since it could have important implications, not only with regard to antisera-induced malformations but because these findings may be applied to malformations induced by chemicals and other environmental influences.