Anti-inflammative effect of glycyrrhizin on rat thermal injury via inhibition of high-mobility group box 1 protein
Anti-inflammative effect of glycyrrhizin on rat thermal injury via inhibition of high-mobility group box 1 protein
复制标题
DOI：
10.1016/j.burns.2014.05.008
复制
发表时间：
2015-03-01
期刊：
影响因子：
2.7
通讯作者：
Tan, Qian
中科院分区：
文献类型：
--
作者：
Shen, Liangyun;Cui, Ziwei;Tan, Qian
目的：甘草甜素（Gly）是细胞外高迁移率族蛋白B1（I-IMGB 1）细胞因子活性的抑制剂，对大鼠脊髓、肝脏、心脏和脑缺血再灌注损伤具有保护作用。方法：24只雄性Sprague-Dawley大鼠（200-250 g）随机分为对照组、烫伤组和Gly治疗组，每组8只。在后两组中，大鼠遭受30%TBSA（全身表面积）全层烫伤。在Gly处理的烧伤组中，在烧伤后立即和在第24小时腹膜内施用胡萝卜素（60 mg/kg）;在媒介物处理的烧伤组中，在烧伤后立即和在第24小时腹膜内施用林格氏溶液（4 ml/kg，作为媒介物）。伤后48 h处死动物。采集主动脉血样本，用ELISA（酶联免疫吸附试验）试剂盒检测肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1 β）。取肺、肝、肾组织，检测HMGB 1 mRNA和蛋白的表达。结果：严重烧伤后48 h血浆TNF-α和IL-1 β水平明显高于对照组（P < 0.001），伤后48 h血浆TNF-α和IL-1 β水平明显高于对照组（P < 0.001）。腹腔注射Gly（60 mg/kg）可显著降低血清TNF-α和IL-1 β水平（P < 0.01）。Gly处理后，上述生化指标均降低，HMGB 1蛋白和mRNA表达水平降低（P < 0.05和P < 0.01）。结论：Gly具有抗炎作用，对烧伤后远隔器官有保护作用。(C)2014 Elsevier Ltd和ISBI。All rights reserved.
Aim: Glycyrrhizin (Gly) has been reported as an inhibitor of extracellular I-IMGB1 (high-mobility group box 1 protein) cytokine's activity, and protects spinal cord, liver, heart and brain against ischemia-reperfusion-induced injury in rats. The purpose of this study was to investigate the protective effect of Gly in rat skin thermal injury model and to elucidate the underlying mechanisms.Methods: Twenty-four male Sprague-Dawley rats (200-250 g) were randomly divided into control group, vehicle-treated and Gly-treated burn groups, each group contained eight animals. In the latter two groups, rats were subjected to 30% TBSA (Total Body Surface Area) full-thickness scald injury. In Gly-treated burn group, glycyrrhizin (60 mg/kg) was administered intraperitoneally immediately after and at 24th hour burn; in vehicle-treated burn group, Ringer's solution (4 ml/kg, as a vehicle) was administered intraperitoneally immediately after and at 24th hour burn. The animals were sacrificed at 48 h after injury. Aortic blood samples were obtained to detect tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) with ELISA (Enzyme-Linked Immuno Sorbent Assay) kits. Lung, liver and kidney tissue samples were collected to determine the expression of HMGB1 mRNA and protein. HMGB1 mRNA level was semiquantitatively measured by Real-Time PCR using beta-actin as an internal standard, and protein expression of HMGBI was determined by Western blot.Results: Severe skin scald injury caused a significant increase in plasma TNF-alpha and IL-1 beta versus the control group (P < 0.001) in 48 h after burns. Intraperitoneal administration of Gly (60 mg/kg) significantly reduced the levels of serum TNF-alpha and IL-1 beta (P < 0.01). Gly treatment reduced these biochemical indices accompanied by lower level of HMGB1 protein (P < 0.05) and mRNA expression (P < 0.01).Conclusion: These results demonstrate that Gly possesses an anti-inflammation effect to protect the remote organs from burn-induced injury. (C) 2014 Elsevier Ltd and ISBI. All rights reserved.