The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.
The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.
复制标题
荧光和光活性蛋白的生长和发光工具箱。
DOI：
10.1016/j.tibs.2016.09.010
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发表时间：
2017-02
影响因子：
13.8
通讯作者：
Tsien RY
中科院分区：
文献类型：
--
作者：
Rodriguez EA;Campbell RE;Lin JY;Lin MZ;Miyawaki A;Palmer AE;Shu X;Zhang J;Tsien RY
在过去的20年里，蛋白质工程已被广泛用于改善和修改荧光蛋白（FP）的基本特性，目的是使其适应各种各样的应用。通过合理设计、基于结构的诱变和无数次定向进化循环（基因多样化，然后选择具有所需特性的克隆）的组合，FP已经被修饰，这些组合共同将特性推向了生物物理和生物化学的极端。在这篇综述中，我们试图提供一个总结，在过去的二十年中已经取得的进展，并在哺乳动物系统中的FP开发和应用的现状进行了广泛的概述。
Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting them for a fantastic range of applications. FPs have been modified by a combination of rational design, structure-based mutagenesis, and countless cycles of directed evolution (gene diversification followed by selection of clones with desired properties) that have collectively pushed the properties to photophysical and biochemical extremes. In this review, we attempt to provide both a summary of the progress that has been made during the past two decades, and a broad overview of the current state of FP development and applications in mammalian systems.