Characterization of microstructure and bio-absorption of the hydroxyapatite ceramics modified by a partial dissolution-precipitation technique using supersonic treatment
Characterization of microstructure and bio-absorption of the hydroxyapatite ceramics modified by a partial dissolution-precipitation technique using supersonic treatment
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DOI：
10.2109/jcersj2.118.535
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发表时间：
2010-06-01
影响因子：
1.1
通讯作者：
Sakamoto, Michiko
中科院分区：
文献类型：
--
作者：
Akazawa, Toshiyuki;Murata, Masaru;Sakamoto, Michiko
采用溶解-沉淀法对商品化羟基磷灰石（HAp）进行改性，制备了部分溶解沉淀型羟基磷灰石（PDP-HAp）陶瓷，该方法包括在298 K下在含有Ca 2+和PO 43-离子或/和Ca 2+和PO 43-离子的1.7-3.4 x 10（-2）N-HNO 3溶液（50 cm（3））中以300 rpm搅拌。并在相同溶液中以120 W和38 kHz进行超声处理。多孔HAp产品的超声处理，这是远远高于通过搅拌，溶解效率急剧增加，随着时间的推移，取决于陶瓷的孔隙率和HNO 3溶液的浓度。对于均匀致密的HAp产品，超声处理20 min可识别微孔的增强和微裂纹的扩展。在搅拌30 min和随后超声处理25 min后，在大孔和微孔中，HAp纳米晶成功地沉积在孔壁表面，其组成比（Ca/P）为1.64-1.66。在298 K和pH 9-10的陶瓷孔隙。将大孔径为50-200 μ m、孔隙率为85- 90%、比表面积为1-2 μ m（2）·g（-1）的PDP-HAp陶瓷植入日本白色家兔股骨内侧髁骨缺损处。在植入后8周和16周，PDP-HAp陶瓷表现出比商业HAp产品更优异的生物吸收和组织亲和性，因为平滑的体液渗透和有效的表面性质用于细胞吸附。所研制的PDP-HAp可作为一种具有良好生物吸收和骨传导特性的生物活性支架材料应用于骨再生治疗。(C)2010日本陶瓷学会版权所有
Commercial products of hydroxyapatite (HAp) were easily modified to obtain partially-dissolved and precipitated HAp (PDP-HAp) ceramics by the specific dissolution-precipitation technique, which involved a stirring of 300 rpm at 298 K in 1.7-3.4 x 10(-2) N-HNO3 solutions (50 cm(3)) containing Ca2+ and PO43- ions or/and a supersonic treatment at 120 W and 38 kHz in the same solutions. The dissolution efficiency of porous HAp products by the supersonic treatment, which was much higher than that by stirring, drastically increased with time, depending on the porosity of ceramics and the concentration of HNO3 solution. For even dense HAp products, enhancement of micro-pores and propagation of micro-cracks were recognized by the supersonic treatment for 20 min. After the stirring of 30 min and the subsequently supersonic treatment of 25 min, HAp nano-crystals with the composition ratio (Ca/P) of 1.64-1.66 were successfully precipitated on the pore wall surface in the macro-pores and micro-pores of the ceramics at 298 K and pH 9-10. The PDP-HAp ceramics that gave macro-pore sizes of 50-200 mu m, porosities of 85-90%, and specific surface areas of 1-2 m(2).g(-1), were implanted into the bone defects at the medial condyle of femur in Japanese white rabbits. At 8 and 16 weeks after the implantation, the PDP-HAp ceramics exhibited more excellent bio-absorption and tissue-affinity than commercial HAp products because of smooth body-fluid-permeation and effective surface nature for cell-adsorption. The PDP-HAp developed can be applied as one of bioactive scaffolds with good bio-absorption and osteoconduction characteristics for bone-regeneration therapy. (C) 2010 The Ceramic Society of Japan All rights reserved