miR-21 and miR-155 are associated with mitotic activity and lesion depth of borderline melanocytic lesions.
miR-21 and miR-155 are associated with mitotic activity and lesion depth of borderline melanocytic lesions.
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DOI：
10.1038/bjc.2011.288
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发表时间：
2011-09-27
影响因子：
8.8
通讯作者：
Carson, W. E., III
中科院分区：
文献类型：
--
作者：
Grignol, V.;Fairchild, E. T.;Zimmerer, J. M.;Lesinski, G. B.;Walker, M. J.;Magro, C. M.;Kacher, J. E.;Karpa, V. I.;Clark, J.;Nuovo, G.;Lehman, A.;Volinia, S.;Agnese, D. M.;Croce, C. M.;Carson, W. E., III
microRNA (miR) 的表达已被证明在许多实体瘤中发生改变，并且正在黑色素瘤中进行探索。一些黑素细胞病变的恶性潜力很难预测。我们假设，边缘黑素细胞增殖中 miR 表达的表征将导致分子谱的鉴定，该分子谱可与已知的预后因素一起使用，以区分具有高恶性潜力的病变。通过实时 PCR 评估黑素细胞病变（良性痣、恶性黑素瘤和交界性黑素细胞肿瘤）的 miR 表达谱。 PCR分析显示，与良性痣相比，原发性皮肤黑色素瘤的miR-21过表达量是8.6倍，miR-155过表达量是良性痣的7.5倍（P<0.0001）。原位杂交证实了这些结果。 miR-21 和 miR-155 在交界性病变内显着过表达（分别为 P=0.0011 和 P=0.0048）。当根据有丝分裂活性和 Breslow 厚度对边界病变进行分类时，miR-21 与有丝分裂活性相关，miR-155 与厚度相关（P<0.025）。在 14 名接受前哨淋巴结活检 (SLNB) 的交界性病变患者中，与 SLNB 阴性病变相比，SLNB 阳性与原发病变中 miR-21 和 miR-155 升高相关。 MicroRNA 表达谱可用于表征非典型黑素细胞病变。
Expression of microRNAs (miRs) has been shown to be altered in many solid tumours and is being explored in melanoma. The malignant potential of some melanocytic lesions is difficult to predict. We hypothesised that characterisation of miR expression in borderline melanocytic proliferations would lead to the identification of a molecular profile that could be used with known prognostic factors to differentiate lesions with high malignant potential. The miR expression profile of melanocytic lesions (benign naevi, malignant melanoma and borderline melanocytic tumours) was evaluated by real-time PCR. PCR analysis revealed primary cutaneous melanomas had an 8.6-fold overexpression of miR-21 and a 7.5-fold overexpression of miR-155 compared with benign naevi (P<0.0001). In situ hybridisation confirmed these results. miR-21 and miR-155 were significantly overexpressed within borderline lesions (P=0.0011 and P=0.0048, respectively). When borderline lesions were categorised by mitotic activity and Breslow thickness, miR-21 was associated with mitotic activity and miR-155 was associated with thickness (P<0.025). Among 14 patients with borderline lesions who underwent sentinel lymph node biopsy (SLNB), positive SLNB was associated with increased miR-21 and miR-155 in the primary lesion compared with lesions with a negative SLNB. MicroRNA expression profiles can be used to characterise atypical melanocytic lesions.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
6.5
作者：
Mueller, Daniel W.;Rehli, Michael;Bosserhoff, Anja K.
通讯作者：
Bosserhoff, Anja K.
DOI：
10.1073/pnas.0602266103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Costinean, S;Zanesi, N;Croce, CM
通讯作者：
Croce, CM
MicroRNA-155 通过靶向细胞因子信号传导 1 基因的抑制子在乳腺癌中发挥 OncomiR 的作用
影响因子：
11.2
作者：
Jiang, Shuai;Zhang, Hong-Wei;Wang, En-Duo
通讯作者：
Wang, En-Duo
影响因子：
5.8
作者：
Nikiforova, Marina N.;Tseng, George C.;Nikiforov, Yuri E.
通讯作者：
Nikiforov, Yuri E.