Bruch's Membrane Thickness and Retinal Pigment Epithelium Cell Density in Experimental Axial Elongation
Bruch's Membrane Thickness and Retinal Pigment Epithelium Cell Density in Experimental Axial Elongation
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实验轴伸长时布鲁赫膜厚度和视网膜色素上皮细胞密度
DOI：
10.1038/s41598-019-43212-8
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发表时间：
2019-04-29
影响因子：
4.6
通讯作者：
Jonas, Jost B.
中科院分区：
文献类型：
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作者：
Dong, Li;Shi, Xu Han;Jonas, Jost B.
为了评估进行性近视眼的解剖学变化，我们对晶状体诱导的眼轴延长的豚鼠眼进行了形态计量学检查。从3-4周龄开始，实验组(n=20只)的豚鼠戴上护目镜5周，形成单侧晶状体诱导的眼轴延长，而对照组20只没有进行干预(眼轴长度：8.91±0.08 mm比8.74±0.07 mm；P<；0.001)。基线5周后，处死动物，摘除眼球。在锯齿缘(P=0.41)、赤道(P=0.41)、赤道与后极中点(P=0.13)或后极(P=0.89)处，Bruch膜厚度与眼轴长度均无显著相关。视网膜色素上皮(RPE)细胞密度在MBEPP(P=0.04；回归系数β=-0.33)和后极(P=0.01；β=-0.40)处随轴长增加而降低。此外，视网膜和巩膜厚度随着眼轴的增加而减小(P=0.01；β=-0.42；P<；0.001；β=-0.64)；后极(P<；0.001；β=-0.51；P<；0.001；β=-0.45)。脉络膜厚度在后极部减少(P<；0.001；β=-0.51)。实验性轴向延长与视网膜、脉络膜和巩膜变薄以及RPE细胞密度降低有关，最明显的是后极部。Bruch膜厚度与轴向伸长率无关。
To assess anatomical changes in eyes with progressive myopia, we morphometrically examined the eyes of guinea pigs with lens-induced axial elongation. Starting at an age of 3-4 weeks, guinea pigs in the experimental group (n = 20 animals) developed unilateral lens-induced axial elongation by wearing goggles for 5 weeks compared to a control group of 20 animals without intervention (axial length: 8.91 +/- 0.08 mm versus 8.74 +/- 0.07 mm; P < 0.001). Five weeks after baseline, the animals were sacrificed, and the eyes enucleated. As measured histomorphometrically, Bruch's membrane thickness was not significantly correlated with axial length in either group at the ora serrata (P = 0.41), equator (P = 0.41), midpoint between equator and posterior pole (MBEPP) (P = 0.13) or posterior pole (P = 0.89). Retinal pigment epithelium (RPE) cell density decreased with longer axial length at the MBEPP (P = 0.04; regression coefficient beta = -0.33) and posterior pole (P = 0.01; beta = -0.40). Additionally, the thickness of the retina and sclera decreased with longer axial length at the MBEPP (P = 0.01; beta = -0.42 and P < 0.001; beta = -0.64, respectively) and posterior pole (P < 0.001; beta = -0.51 and P < 0.001; beta = -0.45, respectively). Choroidal thickness decreased at the posterior pole (P < 0.001; beta = -0.51). Experimental axial elongation was associated with a thinning of the retina, choroid and sclera and a decrease in RPE cell density, most markedly at the posterior pole. Bruch's membrane thickness was not related to axial elongation.