The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine.
The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine.
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DOI：
10.1083/jcb.51.3.752
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发表时间：
1971-12
期刊：
影响因子：
--
通讯作者：
Goldman RD
中科院分区：
文献类型：
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作者：
Goldman RD
在BHK-21/C13成纤维细胞样细胞中观察到5或40µg/ml秋水仙碱存在时微管破裂。在微管缺失的情况下，可以观察到几种形态学和生理效应：(a)细胞从成纤维细胞样转变为上皮样细胞；(b)细胞内正常双折射模式改变，形成核旁双折射丝帽；(c)延时摄影显示，秋水仙碱处理细胞的细胞运动受到抑制，尽管膜褶皱仍然存在。这些结果讨论了微管在培养细胞运动中的具体作用，以及在BHK-21细胞中发现的三种细胞质纤维的可能功能关系。
Microtubule breakdown in the presence of 5 or 40 µg/ml of colchicine is observed in BHK-21/C13 fibroblast-like cells. Several morphological and physiological effects are noted in the absence of microtubules: (a) the cells transform from fibroblast-like to epithelial-like cells; (b) the normal pattern of intracellular birefringence changes and a juxtanuclear cap of birefringent filaments is formed; (c) time-lapse cinematography demonstrates that cell locomotion is inhibited in colchicine-treated cells, even though membrane ruffling persists. The results are discussed in terms of the specific roles of microtubules in cultured cell motility and possible functional relationships of the three types of cytoplasmic fibers seen in BHK-21 cells.