Field Testing of Arsenic in Groundwater Samples of Bangladesh Using a Test Kit Based on Lyophilized Bioreporter Bacteria
Field Testing of Arsenic in Groundwater Samples of Bangladesh Using a Test Kit Based on Lyophilized Bioreporter Bacteria
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DOI：
10.1021/es203511k
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发表时间：
2012-03-20
影响因子：
11.4
通讯作者：
Harms, Hauke
中科院分区：
文献类型：
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作者：
Siegfried, Konrad;Endes, Carola;Harms, Hauke
在孟加拉国六个村庄的实地活动中，应用了一种基于活的冻干细菌生物报告者的检测试剂盒，该试剂盒发射生物发光作为对亚砷酸盐和砷酸盐的反应。地下水中砷的biorreporter现场测量从管威尔斯在实验室进行的相同样品的光谱分析结果令人满意的协议。在物流和材料要求、高样品通量的适用性和废物处理方面，生物报告测试的实用性远远优于作为参考的两种商业化学测试试剂盒。这些活动还表明，当地地下水中的砷具有很大的异质性，这突出表明，使用换井是避免高砷暴露的有效补救措施。
A test kit based on living, lyophilized bacterial bioreporters emitting bioluminescence as a response to arsenite and arsenate was applied during a field campaign in six villages across Bangladesh. Bioreporter field measurements of arsenic in groundwater from tube wells were in satisfying agreement with the results of spectroscopic analyses of the same samples conducted in the lab. The practicability of the bioreporter test in terms of logistics and material requirements, suitability for high sample throughput, and waste disposal was much better than that of two commercial chemical test kits that were included as references. The campaigns furthermore demonstrated large local heterogeneity of arsenic in groundwater, underscoring the use of well switching as an effective remedy to avoid high arsenic exposure.