Comparative histological and immunohistochemical study of sea star tube feet (Echinodermata, Asteroidea)
Comparative histological and immunohistochemical study of sea star tube feet (Echinodermata, Asteroidea)
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DOI：
10.1002/jmor.10187
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发表时间：
2005-03-01
影响因子：
1.5
通讯作者：
Flammang, P
中科院分区：
文献类型：
--
作者：
Santos, R;Haesaerts, D;Flammang, P
海星的粘附是特殊结构的功能，管足或足部是水血管系统的外部附属物。粘性分泌物使小行星管足具有多种功能。事实上，根据所考虑的海星物种，管足可能与运动、固定或挖洞有关。不同的管脚形状通常与这种功能相对应。在这项研究中，我们研究了海星管足形态的可变性及其粘连分泌物组成的可变性。这第二个方面是通过比较免疫组织化学研究提出的抗体针对的粘合材料的替克孕酸Asterias rubens。对星总纲5目10科14种海星的管足进行了研究。组织学研究显示三种主要的管足形态，即旋钮末端、简单盘状末端和强化盘状末端。分析结果表明，管足形态受物种栖息地的影响，但受进化谱系的限制。另一方面，在免疫组织化学中，结果是非常均匀的。在所调查的每个物种中，无论所考虑的分类群，管足形态或功能，或物种栖息地，粘附细胞都有很强的免疫标记。这表明在所有被考虑的物种中，粘接剂是密切相关的，可能共享许多相同的分子，或者至少在它们的成分上有许多相同的表位。中国生物医学工程学报，26(3):559 - 559,2005。(C) 2004 Wiley-Liss, Inc。
Adhesion in sea stars is the function of specialized structures, the tube feet or podia, which are the external appendages of the water-vascular system. Adhesive secretions allow asteroid tube feet to perform multiple functions. Indeed, according to the sea star species considered, the tube feet may be involved in locomotion, fixation, or burrowing. Different tube foot shapes usually correspond to this variety of function. In this study, we investigated the variability of the morphology of sea star tube feet as well as the variability of the composition of their adhesive secretions. This second aspect was addressed by a comparative immunohistochemical study using antibodies raised against the adhesive material of the forcipulatid Asterias rubens. The tube feet from 14 sea star species representing five orders and 10 families of the Class Asteroidea were examined. The histological study revealed three main tube foot morphotypes, i.e., knob-ending, simple disc-ending, and reinforced disc-ending. Analysis of the results suggests that tube foot morphology is influenced by species habitat, but within limits imposed by the evolutionary lineage. In immunohistochemistry, on the other hand, the results were very homogeneous. In every species investigated there was a very strong immunolabeling of the adhesive cells, independently of the taxon considered, of the tube foot morphotype or function, or of the species habitat. This indicates that the adhesives in all the species considered are closely related, probably sharing many identical molecules or, at least, many identical epitopes on their constituents. J. Morphol. 263: 259-269,2005. (C) 2004 Wiley-Liss, Inc.